Acute toxicological evaluation of AT-533 and AT-533 gel in Sprague-Dawley rats

BMC Pharmacology and Toxicology

Research

Lishan Zhong¹  Cui-fang Ye¹  Chen Huang¹  Zhe Ren¹  Yifei Wang²  Yanting Wu²  Kaisheng Liu³ 

[1] Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China;Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China;Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China;Department of Cell Biology, College of Life Science and Technology, Jinan University, Guangzhou, China;Guangdong Province Key Laboratory of Bioengineering Medicine, Guangzhou, China;Guangdong Provincial biotechnology drug and Engineering Technology Research Center, Guangzhou, China;Guangzhou (Jinan) Biomedical Research and Development Center Co. Ltd, Guangzhou, China;Guangdong Provincial Clinical Research Center for Geriatrics, Shenzhen Clinical Research Center for Geriatrics,Shenzhen People’s Hospital, The Second Clinical Medical College, Jinan University, Shenzhen, Guangdong, China;Shenzhen People’s Hospital, The First Affiliated Hospital, Southern University of Science and Technology, Shenzhen, Guangdong, China;

关键词: AT-533;    AT-533 gel;    Acute toxicity;    Sprague-Dawley rats;   

DOI  :  10.1186/s40360-023-00696-5

 received in 2023-04-20, accepted in 2023-10-10,  发布年份 2023

来源: Springer

【 摘 要 】

BackgroundAT-533 is a novel heat shock protein 90 inhibitor that exerting anti-inflammatory, antiviral, and antitumor efficacy. Furthermore, the gel made of AT-533 as raw material named AT-533 gel has the function of repairing keratitis and dermatitis caused by herpes virus infection. However, the acute safety evaluation of AT-533 and AT-533 gel has not been conducted.Methods and resultsHerein, we performed acute toxicological studies of AT-533 and AT-533 gel in Sprague-Dawley rats. Fifteen-day acute toxicity study of AT-533 was conducted in both male and female Sprague-Dawley rats at doses of 5, 50, 250 and 500 mg/kg and AT-533 gel at 5 g/kg in the study. During experiment, food consumption and mortality were observed and body weight, hematology, serum biochemistry and histopathological assessment of rats were carried out. No abnormal changes were observed in rats percutaneously treated with AT-533 at 5 mg/kg and 50 mg/kg and AT-533 gel. However, loss of appetite and body weight, adverse reactions, toxicologically relevant alterations in hematology and biochemistry were found in rats percutaneously treated with AT-533 at 250 mg/kg and 500 mg/kg during 15-day acute dermic toxicity study.ConclusionsThe aforementioned results suggested that the LD50 of AT-533 is 228.382 mg/kg and the LD50 of AT-533 gel is greater than 5 g/kg. These findings indicated that AT-533 is non-toxic in rats when the dose less than 50 mg/kg and AT-533 gel can be considered a gel with no toxicity at doses less than 5 g/kg.

【 授权许可】

CC BY   
© BioMed Central Ltd., part of Springer Nature 2023

【 图 表 】

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