Molecular pathways underlying inhibitory effect of antimicrobial peptide Nal-P-113 on bacteria biofilms formation of Porphyromonas gingivalis W83 by DNA microarray

BMC Microbiology

Research Article

Hong-yan Wang¹  Li-si Tan¹  Li Lin¹  Ya-ping Pan¹  Jya-Wei Cheng²  Hui-Yuan Yu² 

[1] Department of Periodontics, School of Stomatology, China Medical University, 110002, Shenyang, China;Institute of Biotechnology and Department of Medical Science, National Tsing Hua University, 300, Hsinchu, Taiwan;

关键词: Porphyromonas gingivalis;    Nal-P-113;    Microarray;    Biofilms;   

DOI  :  10.1186/s12866-017-0948-z

 received in 2016-09-26, accepted in 2017-02-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWound-related infection remains a major challenge for health professionals. One disadvantage in conventional antibiotics is their inability to penetrate biofilms, the main protective strategy for bacteria to evade irradiation. Previously, we have shown that synthetic antimicrobial peptides could inhibit bacterial biofilms formation.ResultsIn this study, we first delineated how Nal-P-113, a novel antimicrobial peptide, exerted its inhibitory effects on Porphyromonas gingivalis W83 biofilms formation at a low concentration. Secondly, we performed gene expression profiling and validated that Nal-P-113 at a low dose significantly down-regulated genes related to mobile and extrachromosomal element functions, transport and binding proteins in Porphyromonas gingivalis W83.ConclusionsThese findings suggest that Nal-P-113 at low dose is sufficient to inhibit the formation of biofilms although Porphyromonas gingivalis W83 may maintain its survival in the oral cavity. The newly discovered molecular pathways may add the knowledge of developing a new strategy to target bacterial infections in combination with current first-line treatment in periodontitis.

【 授权许可】

CC BY   
© The Author(s). 2017