Identification of Molecular and Cellular Responses of Desulfovibrio vulgaris Biofilms under Culture Conditions Relevant to Field Conditions for Bioreduction of Toxic Metals and Radionuclides

Wall, Judy D.

University of Missouri (System). Curators.

关键词: Biofilms;    Radioisotopes Desulfovibrio;    Chromate Reduction;    Genes;    Carbohydrates;   

DOI  :  10.2172/1015868
RP-ID  :  DOEER64126 Final Report
RP-ID  :  FG02-05ER64126
RP-ID  :  1015868

美国|英语

来源: UNT Digital Library

【 摘 要 】

Our findings demonstrated that D. vulgaris surface-adhered populations produce extracellular structures, and that that the cells have altered carbon and energy flux compared to planktonic cells.Biofilms did not have greatly increased carbohydrate accumulation.Interestingly genes present on the native plasmid found in D. vulgaris Hildenborough were necessary for wild type biofilm formation.In addition,extracellular appendages dependent on functions or proteins encoded by flaG or fliA also contributed to biofilm formation.Studies with SRB biofilms have indicated that the reduction and precipitation of metals can occur within the biofilm matrix; however, little work has been done to elucidate the physiological state of surface-adhered cells during metal reduction (Cr6+, U6+) and how this process is affected by nutrient feed levels (i.e., the stimulant).