Antibacterial properties of chicken intestinal phospholipase A2

Lipids in Health and Disease

Research

Yassine Ben Ali¹  Youssef Gargouri¹  Aida Karray¹  Sofiane Bezzine¹ 

[1]Laboratoire de Biochimie et de Génie Enzymatique des Lipases, University of Sfax, ENIS Route de Soukra, 3038, Sfax, Tunisia

关键词: Bactericidal Activity;    Lysozyme;    Bacillus Subtilis;    Bacillus Cereus;    Positive Bacterium;   

DOI  :  10.1186/1476-511X-10-4

 received in 2010-11-01, accepted in 2011-01-12,  发布年份 2011

来源: Springer

【 摘 要 】

BackgroundThe presence of chicken group-IIA PLA2 (ChPLA2-IIA) in the intestinal secretion suggests that this enzyme plays an important role in systemic bactericidal defence. We have analyzed the bactericidal activity of purified ChPLA2-IIA, on several gram-positive and gram-negative bacteria by using the diffusion well and dilution methods.ResultsChPLA2-IIA displays potent bactericidal activity against gram-positive bacteria but lacks bactericidal activity against gram negative ones. We have also demonstrated a synergic action of ChPLA2-IIA with lysozyme when added to the bacteria culture prior to ChPLA2-IIA. The bactericidal efficiency of ChPLA2-IIA was shown to be dependent upon the presence of calcium ions and then a correlation could be made to its hydrolytic activity of membrane phospholipids. Interestingly ChPLA2-IIA displays a higher dependence to Ca2+ ions than to Mg2+ions.ConclusionWe conclude that the main physiological role of ChPLA2-IIA could be the defence of the intestine against bacterial invasions.

【 授权许可】

Unknown   
© Karray et al; licensee BioMed Central Ltd. 2011. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.