Comparison of host response mechanisms evoked by extended spectrum beta lactamase (ESBL)- and non-ESBL-producing uropathogenic E. coli

BMC Microbiology

Research Article

Isak Demirel¹  Katarina Persson¹  Annica Kinnunen¹  Anna Önnberg²  Bo Söderquist² 

[1] Faculty of Medicine and Health, Örebro University, Örebro, Sweden;Faculty of Medicine and Health, Örebro University, Örebro, Sweden;Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden;

关键词: Extended spectrum beta-lactamases;    Urinary tract infections;    Renal epithelial cells;    Polymorphonucleated leukocytes;    E. coli;   

DOI  :  10.1186/1471-2180-13-181

 received in 2013-01-14, accepted in 2013-07-31,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundInfections caused by extended spectrum beta-lactamases (ESBL)-producing bacteria have been emerging worldwide and the majority of ESBL-producing E. coli strains are isolated from patients with urinary tracts infections. The purpose of this study was to compare the host-response mechanisms in human polymorphonucleated leukocytes (PMN) and renal epithelial cells when stimulated by ESBL- or non-ESBL-producing uropathogenic E. coli (UPEC) isolates. The host-pathogen interaction of these ESBL-producing strains in the urinary tract is not well studied.ResultsThe ability of ESBL strains to evoke ROS-production from PMN cells was significantly higher than that of the non-ESBL strains. The growth of ESBL strains was slightly suppressed in the presence of PMN compared to non-ESBL strains after 30 min and 2 h, but the opposite was observed after 5 and 6 h. The number of migrating PMN was significantly higher in response to ESBL strains compared to non-ESBL strains. Stimulation of A498 cells with ESBL strains elicited lower production of IL-6 and IL-8 compared to non-ESBL strains.ConclusionSignificant differences in host-response mechanisms were identified when host cells were stimulated by ESBL- or non-ESBL producing strains. The obtained results on the early interactions of ESBL-producing strains with the host immune system may provide valuable information for management of these infections.

【 授权许可】

CC BY   
© Demirel et al.; licensee BioMed Central Ltd. 2013