BMC Microbiology | |
Comparison of host response mechanisms evoked by extended spectrum beta lactamase (ESBL)- and non-ESBL-producing uropathogenic E. coli | |
Research Article | |
Isak Demirel1  Katarina Persson1  Annica Kinnunen1  Anna Önnberg2  Bo Söderquist2  | |
[1] Faculty of Medicine and Health, Örebro University, Örebro, Sweden;Faculty of Medicine and Health, Örebro University, Örebro, Sweden;Department of Laboratory Medicine, Clinical Microbiology, Örebro University Hospital, Örebro, Sweden; | |
关键词: Extended spectrum beta-lactamases; Urinary tract infections; Renal epithelial cells; Polymorphonucleated leukocytes; E. coli; | |
DOI : 10.1186/1471-2180-13-181 | |
received in 2013-01-14, accepted in 2013-07-31, 发布年份 2013 | |
来源: Springer | |
【 摘 要 】
BackgroundInfections caused by extended spectrum beta-lactamases (ESBL)-producing bacteria have been emerging worldwide and the majority of ESBL-producing E. coli strains are isolated from patients with urinary tracts infections. The purpose of this study was to compare the host-response mechanisms in human polymorphonucleated leukocytes (PMN) and renal epithelial cells when stimulated by ESBL- or non-ESBL-producing uropathogenic E. coli (UPEC) isolates. The host-pathogen interaction of these ESBL-producing strains in the urinary tract is not well studied.ResultsThe ability of ESBL strains to evoke ROS-production from PMN cells was significantly higher than that of the non-ESBL strains. The growth of ESBL strains was slightly suppressed in the presence of PMN compared to non-ESBL strains after 30 min and 2 h, but the opposite was observed after 5 and 6 h. The number of migrating PMN was significantly higher in response to ESBL strains compared to non-ESBL strains. Stimulation of A498 cells with ESBL strains elicited lower production of IL-6 and IL-8 compared to non-ESBL strains.ConclusionSignificant differences in host-response mechanisms were identified when host cells were stimulated by ESBL- or non-ESBL producing strains. The obtained results on the early interactions of ESBL-producing strains with the host immune system may provide valuable information for management of these infections.
【 授权许可】
CC BY
© Demirel et al.; licensee BioMed Central Ltd. 2013
【 预 览 】
Files | Size | Format | View |
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RO202311097783127ZK.pdf | 669KB | download |
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