Uncovering by Atomic Force Microscopy of an original circular structure at the yeast cell surface in response to heat shock

BMC Biology

Research Article

Flavien Pillet¹  Stéphane Lemonier²  Etienne Dague²  Cécile Formosa³  Marion Schiavone⁴  Jean Marie Francois⁵  Hélène Martin-Yken⁵ 

[1] CNRS, LAAS, 7 avenue du colonel Roche, F-31077, Toulouse, France;Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France;CNRS, LAAS, 7 avenue du colonel Roche, F-31077, Toulouse, France;Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France;CNRS, ITAV-USR 3505, F31106, Toulouse, France;CNRS, LAAS, 7 avenue du colonel Roche, F-31077, Toulouse, France;Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France;CNRS, UMR 7565, SRSMC, Vandoeuvre-lès-Nancy, France;Université de Lorraine, UMR 7565, Faculté de Pharmacie, Nancy, France;CNRS, LAAS, 7 avenue du colonel Roche, F-31077, Toulouse, France;Université de Toulouse, UPS, INSA, INP, ISAE, LAAS, F-31077, Toulouse, France;Université de Toulouse, INSA, UPS, INP, 135 avenue de Rangueil, F-31077, Toulouse, France;INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31077, Toulouse, France;CNRS, UMR5504, F-31400, Toulouse, France;Université de Toulouse, INSA, UPS, INP, 135 avenue de Rangueil, F-31077, Toulouse, France;INRA, UMR792 Ingénierie des Systèmes Biologiques et des Procédés, F-31077, Toulouse, France;CNRS, UMR5504, F-31400, Toulouse, France;

关键词: Atomic Force Microscopy (AFM);    Saccharomyces cerevisiae;    Heat-shock;    Cell wall;    Chitin;    Budding;   

DOI  :  10.1186/1741-7007-12-6

 received in 2013-12-09, accepted in 2014-01-10,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAtomic Force Microscopy (AFM) is a polyvalent tool that allows biological and mechanical studies of full living microorganisms, and therefore the comprehension of molecular mechanisms at the nanoscale level. By combining AFM with genetical and biochemical methods, we explored the biophysical response of the yeast Saccharomyces cerevisiae to a temperature stress from 30°C to 42°C during 1 h.ResultsWe report for the first time the formation of an unprecedented circular structure at the cell surface that takes its origin at a single punctuate source and propagates in a concentric manner to reach a diameter of 2–3 μm at least, thus significantly greater than a bud scar. Concomitantly, the cell wall stiffness determined by the Young’s Modulus of heat stressed cells increased two fold with a concurrent increase of chitin. This heat-induced circular structure was not found either in wsc1Δ or bck1Δ mutants that are defective in the CWI signaling pathway, nor in chs1Δ, chs3Δ and bni1Δ mutant cells, reported to be deficient in the proper budding process. It was also abolished in the presence of latrunculin A, a toxin known to destabilize actin cytoskeleton.ConclusionsOur results suggest that this singular morphological event occurring at the cell surface is due to a dysfunction in the budding machinery caused by the heat shock and that this phenomenon is under the control of the CWI pathway.

【 授权许可】

Unknown   
© Pillet et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.