In vivo assessment of arterial stiffness in the isoflurane anesthetized spontaneously hypertensive rat

Cardiovascular Ultrasound

Research

Andrew B Casabianca¹  Samer J Khouri²  Eric E Morgan³  Andrea L Nestor Kalinoski⁴ 

[1] Department of Anesthesiology, University of Toledo, College of Medicine and Life Sciences, 3000 Arlington Ave, 43614, Toledo, OH, USA;Department of Medicine, University of Toledo, College of Medicine and Life Sciences, 3000 Arlington Ave, 43614, Toledo, OH, USA;Department of Physiology and Pharmacology, University of Toledo, College of Medicine and Life Sciences, 3000 Arlington Ave, 43614, Toledo, OH, USA;The University of Toledo Advanced Microscopy & Imaging Center, University of Toledo, College of Medicine and Life Sciences, 3000 Arlington Ave, 43614, Toledo, OH, USA;Department of Surgery, University of Toledo, College of Medicine and Life Sciences, 3000 Arlington Ave, 43614, Toledo, OH, USA;The University of Toledo Advanced Microscopy & Imaging Center, University of Toledo, College of Medicine and Life Sciences, 3000 Arlington Ave, 43614, Toledo, OH, USA;

关键词: Blood pressure;    Arterial stiffness;    Pulse wave velocity;    Arterial elasticity index;    Doppler;    Ultrasound;    Isoflurane;    Anesthesia;    SHR;    Rat;   

DOI  :  10.1186/1476-7120-12-37

 received in 2014-03-12, accepted in 2014-09-12,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundRodent models are increasingly used to study the development and progression of arterial stiffness. Both the non-invasive Doppler derived Pulse Wave Velocity (PWV) and the invasively determined arterial elastance index (EaI) have been used to assess arterial stiffness in rats and mice, but the need for anesthetic agents to make these in vivo estimates may limit their utility. Thus, we sought to determine: 1) if known differences in arterial stiffness in spontaneously hypertensive rats (SHR) are detectable by PWV and EaI measurements when made under isoflurane anesthesia, and 2) if these two uniquely acquired assessments of arterial elasticity correlate.MethodsWe obtained PWV and EaI measurements in isoflurane anesthetized young and old SHRs, which are known to have significant differences in arterial stiffness. Doppler pulse waves were recorded from carotid and iliac arteries and the distance (D) between probe applantation sites was recorded. Simultaneously, an EKG was obtained, and the time intervals between the R-wave of the EKG to the foot of the Doppler waveforms were measured and averaged over three cardiac cycles. Pulse-transit time (T) of the carotid to iliac artery was determined, and PWV was calculated as Distance (D)/Time (T), where D = the distance from the carotid to the iliac notch and T = (R to iliac foot) - (R to carotid foot). EaI was subsequently determined from pressure volumes loops obtained via left ventricle catheterization.ResultsPWV and EaI were found to be significantly faster in the older rats (13.2 ± 2.0 vs. 8.0 ± 0.8 m/sec, p < 0.001; 120 ± 20 vs. 97 ± 16 mmHg/μl/g, p <0.05). Bland-Altman analyses of intra- and inter-observer measures demonstrate a statistically significant relationship between readings (p < 0.0001). PWV and EaI measurements were found to be significantly and positively correlated with a correlation coefficient of 0.53 (p < 0.05).ConclusionOur study suggests that isoflurane administration does not limit Doppler PWV or EaI measures in their ability to provide accurate, in vivo assessments of relative arterial stiffness in isoflurane anesthetised SHR rats. Furthermore, PWV data obtained in these rats correlate well with invasively determined EaI.

【 授权许可】

Unknown   
© Morgan et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.