Insights into the human mesenchymal stromal/stem cell identity through integrative transcriptomic profiling

BMC Genomics

Research Article

Javier De Las Rivas¹  Beatriz Roson-Burgo²  Fermin Sanchez-Guijo³  Consuelo Del Cañizo³ 

[1] Bioinformatics and Functional Genomics Group, Cancer Research Center (IBMCC, CSIC/USAL) and IBSAL, Consejo Superior de Investigaciones Cientificas (CSIC), Salamanca, Spain;Bioinformatics and Functional Genomics Group, Cancer Research Center (IBMCC, CSIC/USAL) and IBSAL, Consejo Superior de Investigaciones Cientificas (CSIC), Salamanca, Spain;Hematology Department, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain;Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Salamanca, Spain;Hematology Department, IBSAL-Hospital Universitario de Salamanca, Salamanca, Spain;Centro en Red de Medicina Regenerativa y Terapia Celular de Castilla y León, Salamanca, Spain;

关键词: Stromal cells;    Mesenchymal stem cells;    Placenta;    Bone marrow;    Adipose tissue;    Human gene expression;    Bioinformatic meta-analysis;    Cytokines;    CD marker;   

DOI  :  10.1186/s12864-016-3230-0

 received in 2016-01-05, accepted in 2016-11-01,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMesenchymal Stromal/Stem Cells (MSCs), isolated under the criteria established by the ISCT, still have a poorly characterized phenotype that is difficult to distinguish from similar cell populations. Although the field of transcriptomics and functional genomics has quickly grown in the last decade, a deep comparative analysis of human MSCs expression profiles in a meaningful cellular context has not been yet performed. There is also a need to find a well-defined MSCs gene-signature because many recent biomedical studies show that key cellular interaction processes (i.e. inmuno-modulation, cellular cross-talk, cellular maintenance, differentiation, epithelial-mesenchymal transition) are dependent on the mesenchymal stem cells within the stromal niche.ResultsIn this work we define a core mesenchymal lineage signature of 489 genes based on a deep comparative analysis of multiple transcriptomic expression data series that comprise: (i) MSCs of different tissue origins; (ii) MSCs in different states of commitment; (iii) other related non-mesenchymal human cell types. The work integrates several public datasets, as well as de-novo produced microarray and RNA-Seq datasets. The results present tissue-specific signatures for adipose tissue, chorionic placenta, and bone marrow MSCs, as well as for dermal fibroblasts; providing a better definition of the relationship between fibroblasts and MSCs. Finally, novel CD marker patterns and cytokine-receptor profiles are unravelled, especially for BM-MSCs; with MCAM (CD146) revealed as a prevalent marker in this subtype of MSCs.ConclusionsThe improved biomolecular characterization and the released genome-wide expression signatures of human MSCs provide a comprehensive new resource that can drive further functional studies and redesigned cell therapy applications.

【 授权许可】

CC BY   
© The Author(s). 2016