Production of reactive oxygen species and wound-induced resistance in Arabidopsis thaliana against Botrytis cinereaare preceded and depend on a burst of calcium

BMC Plant Biology

Research Article

Alex Costa¹  Emna Beneloujaephajri²  Jean-Pierre Métraux²  Floriane L’Haridon²  Matteo Binda³ 

[1] Department of Biosciences, University of Milan, via G. Celoria 26, 20133, Milan, Italy;Department of biology, University of Fribourg, Ch. du Musée 10, 1700, Fribourg, Switzerland;Department of biology, University of Fribourg, Ch. du Musée 10, 1700, Fribourg, Switzerland;Medion Grifols Diagnostics AG, Bonnstrasse 9, 3186, Düdingen, Switzerland;

关键词: Arabidopsis thaliana;    Botrytis cinerea;    Cuticle;    Wounding;    Resistance;    Calcium;    ROS;   

DOI  :  10.1186/1471-2229-13-160

 received in 2013-07-04, accepted in 2013-10-13,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundWounded leaves of Arabidopsis thaliana produce reactive oxygen species (ROS) within minutes after wounding and become resistant to the pathogenic fungus Botrytis cinerea at a local level. This fast response of the plants to the wound is called wound-induced resistance (WIR). However the molecular mechanisms of this response and the signal cascade between the wound and ROS production are still largely unknown. Calcium is a conserved signal and it is involved in many abiotic stress responses in plants, furthermore, calcium pathways act very fast.ResultsThe results of this study show that leaves treated with calcium channels inhibitors (verapamil) or calcium chelators (oxalate and EGTA) are impaired in ROS production. Moreover, leaves treated with verapamil, EGTA or oxalate were more susceptible to B. cinerea after wounding. The intracellular measurements of calcium changes indicated quick but transient calcium dynamics taking place few seconds after wounding in cells neighbouring the wound site. This change in the cytosolic calcium was followed in the same region by a more stable ROS burst.ConclusionsThese data further extend our knowledge on the connection between wounding, calcium influx and ROS production. Moreover they provide for the first time the evidence that, following wounding, calcium changes precede a burst in ROS in the same location.

【 授权许可】

Unknown   
© Beneloujaephajri et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.