Short oligogalacturonides induce pathogen resistance-associated gene expression in Arabidopsis thaliana

BMC Plant Biology

Research Article

Minna Pirhonen¹  E. Tapio Palva²  Nina Sipari²  Tarja Kariola²  Pär Davidsson²  Martin Broberg³ 

[1] Department of Agricultural Sciences, University of Helsinki, Helsinki, Finland;Department of Biosciences, Division of Genetics, University of Helsinki, Helsinki, Finland;School of Biological Sciences, Bangor University, Bangor, Wales, UK;

关键词: Plant signaling;    Arabidopsis thaliana;    Oligogalacturonides;    OG;    Trimers;    Transcriptomics;    Defense induction;    Growth inhibition;    Disease resistance;    Pectobacterium carotovorum;    Botrytis cinerea;   

DOI  :  10.1186/s12870-016-0959-1

 received in 2016-06-15, accepted in 2016-12-20,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundOligogalacturonides (OGs) are important components of damage-associated molecular pattern (DAMP) signaling and influence growth regulation in plants. Recent studies have focused on the impact of long OGs (degree of polymerization (DP) from 10–15), demonstrating the induction of plant defense signaling resulting in enhanced defenses to necrotrophic pathogens. To clarify the role of trimers (trimeric OGs, DP3) in DAMP signaling and their impact on plant growth regulation, we performed a transcriptomic analysis through the RNA sequencing of Arabidopsis thaliana exposed to trimers.ResultsThe transcriptomic data from trimer-treated Arabidopsis seedlings indicate a clear activation of genes involved in defense signaling, phytohormone signaling and a down-regulation of genes involved in processes related to growth regulation and development. This is further accompanied with improved defenses against necrotrophic pathogens triggered by the trimer treatment, indicating that short OGs have a clear impact on plant responses, similar to those described for long OGs.ConclusionsOur results demonstrate that trimers are indeed active elicitors of plant defenses. This is clearly indicated by the up-regulation of genes associated with plant defense signaling, accompanied with improved defenses against necrotrophic pathogens. Moreover, trimers simultaneously trigger a clear down-regulation of genes and gene sets associated with growth and development, leading to stunted seedling growth in Arabidopsis.

【 授权许可】

CC BY   
© The Author(s). 2017