Curcuma DMSO extracts and curcumin exhibit an anti-inflammatory and anti-catabolic effect on human intervertebral disc cells, possibly by influencing TLR2 expression and JNK activity

Journal of Inflammation

Research

Oliver Hausmann¹  Babette Klopprogge²  Alexia N Gloess²  Lilian Quero³  Karin Wuertz⁴  Marina Klawitter⁵  Norbert Boos⁶  Juergen Klasen⁷ 

[1] Department of Neurosurgery, Clinic St. Anna, Lucerne, Switzerland;Institute of Chemistry and Biological Chemistry, Zurich University of Applied Sciences, Waedenswil, Switzerland;Spine Research Group, Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland;Spine Research Group, Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland;AOSpine Research Network, Duebendorf, Switzerland;Institute of Physiology and Zurich Center for Integrative Human Physiology (ZIHP), University of Zurich, Zurich, Switzerland;Institute for Biomechanics, Swiss Federal Institute of Technology (ETH), Zurich, Switzerland;Institute for Biomechanics, Swiss Federal Institute of Technology (ETH) Zurich, Schafmattstrasse 30, 8093, Zurich, Switzerland;Spine Research Group, Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland;Bone and Stem Cell Research Group, Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland;Spine Research Group, Competence Center for Applied Biotechnology and Molecular Medicine, University of Zurich, Zurich, Switzerland;University Hospital Balgrist, University of Zurich, Zurich, Switzerland;AOSpine Research Network, Duebendorf, Switzerland;University Hospital Balgrist, University of Zurich, Zurich, Switzerland;

关键词: Human intervertebral disc cells;    Curcumin;    Curcuma;    Proinflammatory cytokines;    Matrix degrading enzymes;    NF-κB;    Toll-like receptors;    MAP kinase;    Back pain;    HPLC/MS;   

DOI  :  10.1186/1476-9255-9-29

 received in 2011-12-04, accepted in 2012-07-15,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundAs proinflammatory cytokines seem to play a role in discogenic back pain, substances exhibiting anti-inflammatory effects on intervertebral disc cells may be used as minimal-invasive therapeutics for intradiscal/epidural injection. The purpose of this study was to investigate the anti-inflammatory and anti-catabolic potential of curcuma, which has been used in the Indian Ayurvedic medicine to treat multiple ailments for a long time.MethodsHuman disc cells were treated with IL-1β to induce an inflammatory/catabolic cascade. Different extracts of curcuma as well as curcumin (= a component selected based on results with curcuma extracts and HPLC/MS analysis) were tested for their ability to reduce mRNA expression of proinflammatory cytokines and matrix degrading enzymes after 6 hours (real-time RT-PCR), followed by analysis of typical inflammatory signaling mechanisms such as NF-κB (Western Blot, Transcription Factor Assay), MAP kinases (Western Blot) and Toll-like receptors (real-time RT-PCR). Quantitative data was statistically analyzed using a Mann Whitney U test with a significance level of p < 0.05 (two-tailed).ResultsResults indicate that the curcuma DMSO extract significantly reduced levels of IL-6, MMP1, MMP3 and MMP13. The DMSO-soluble component curcumin, whose occurrence within the DMSO extract was verified by HPLC/MS, reduced levels of IL-1β, IL-6, IL-8, MMP1, MMP3 and MMP13 and both caused an up-regulation of TNF-α. Pathway analysis indicated that curcumin did not show involvement of NF-κB, but down-regulated TLR2 expression and inhibited the MAP kinase JNK while activating p38 and ERK.ConclusionsBased on its anti-inflammatory and anti-catabolic effects, intradiscal injection of curcumin may be an attractive treatment alternative. However, whether the anti-inflammatory properties in vitro lead to analgesia in vivo will need to be confirmed in an appropriate animal model.

【 授权许可】

Unknown   
© Klawitter et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.