Efficient green silver nanoparticles-antibiotic combinations against antibiotic-resistant bacteria

AMB Express

Original Article

Siyab Alam¹  Tariq Khan¹  Irfan Ullah¹  Urooj Amin¹  Muhammad Adil²  Mian Muhammad³  Muti Ullah⁴  Asma Rehman⁵ 

[1] Department of Biotechnology, University of Malakand, 18800, Chakdara, Dir Lower, Pakistan;Department of Biotechnology, University of Malakand, 18800, Chakdara, Dir Lower, Pakistan;CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety and CAS Center for Excellence in Nanoscience, National Center for Nanoscience and Technology, University of Chinese Academy of Sciences, Beijing, China;Department of Chemistry, University of Malakand, 18800, Chakdara, Dir Lower, Pakistan;Institute of Pathology and Diagnostic Medicine, Khyber Medical University, Peshawar, Pakistan;Nanobiotechnology Group, Industrial Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE), Faisalabad, Punjab, Pakistan;

关键词: Silver nanoparticles;    Cephalosporins;    Antibiotics;    Mentha longifolia;    Antibiotic resistant bacteria;   

DOI  :  10.1186/s13568-023-01619-7

 received in 2023-01-24, accepted in 2023-10-03,  发布年份 2023

来源: Springer

【 摘 要 】

Antibiotic-resistant bacterial strains and the consequent surge in infections caused by them have become major public health concerns. Silver nanoparticles (AgNPs) exhibit antibacterial properties and have wide applications in biomedical sciences. In this study, AgNPs were synthesized in the presence of antibiotics: Ceftazidime (Cft), Cefotaxime (Cef), Ceftriaxone (Cfx), and Cefepime (Cpm), along with the extract of Mentha longifolia. Mentha longifolia-based AgNPs were kept as the control for all experiments. The associated metabolites, structural properties, surface charges, and antibacterial activity of the AgNPs were also evaluated. Overall, a blue-shift of SPR peaks was observed for control AgNPs (λmax = 421 nm, 422 nm, 426 nm, and 406 nm for Cft-AgNPs, Cef-AgNPs, Cfx-AgNPs, and Cpm-AgNPs, respectively), compared to the control (λmax = 438 nm). Fourier-transform infrared spectroscopy showed that antibiotic-based AgNPs had distinct peaks that corresponded to the respective antibiotics, which were not observed in the control. XRD analysis showed that there were observed changes in crystallinity in antibiotic-based AgNPs compared to the control. TEM images revealed that all samples had spherical nanoparticles with different sizes and distributions compared to the control. The Zeta potential for extract-based AgNPs was − 33.6 mV, compared to -19.6 mV for Cft-AgNPs, -2 mV for Cef-AgNPs, -21.1 mV for Cfx-AgNPs, and − 24.2 mV for Cpm-AgNPs. The increase in the PDI value for antibiotic-based AgNPs also showed a highly polydisperse distribution. However, the antibiotic-AgNPs conjugates showed significantly higher activity against pathogenic bacteria. The addition of antibiotics to AgNPs brought significant changes in structural properties and antibacterial activities.

【 授权许可】

CC BY   
© Springer-Verlag GmbH Germany, part of Springer Nature 2023

【 图 表 】

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