期刊论文详细信息
BMC Infectious Diseases
Distinct genotypic profiles of the two major clades of Mycobacterium africanum
Research Article
Richard C Huard1  John L Ho2  Adalberto R Santos3  Sidra E Gonçalves Vasconcellos3  Philip N Suffys3  Kristin Kremer4  Stefan Niemann5 
[1] Clinical Microbiology Service and the Department of Pathology, New York-Presbyterian Hospital, Columbia University Medical Center, 622 West 168th Street, New York City, NY, USA;Division of International Medicine and Infectious Diseases, Department of Medicine, Joan and Sanford I. Weill Medical College of Cornell University, 1300 York Avenue, New York City, NY, USA;Laboratory of Molecular Biology Applied to Mycobacteria, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Avenida Brasil 4365, 21040-900, ManguinhosRio de Janeiro, Brazil;Mycobacteria Reference Laboratory, (CIb/LIS), National Institute for Public Health and the Environment, 3720, Bilthoven, The Netherlands;National Reference Center for Mycobacteria, Forschungszentrum, Parkallee 18, D-23845, Borstel, Germany;
关键词: Tuberculosis;    Mycobacterium Bovis;    Phylogenetic Marker;    Tuberculosis Strain;    West African Country;   
DOI  :  10.1186/1471-2334-10-80
 received in 2009-12-01, accepted in 2010-03-29,  发布年份 2010
来源: Springer
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【 摘 要 】

BackgroundMycobacterium tuberculosis is the principal etiologic agent of human tuberculosis (TB) and a member of the M. tuberculosis complex (MTC). Additional MTC species that cause TB in humans and other mammals include Mycobacterium africanum and Mycobacterium bovis. One result of studies interrogating recently identified MTC phylogenetic markers has been the recognition of at least two distinct lineages of M. africanum, known as West African-1 and West African-2.MethodsWe screened a blinded non-random set of MTC strains isolated from TB patients in Ghana (n = 47) for known chromosomal region-of-difference (RD) loci and single nucleotide polymorphisms (SNPs). A MTC PCR-typing panel, single-target standard PCR, multi-primer PCR, PCR-restriction fragment analysis, and sequence analysis of amplified products were among the methods utilized for the comparative evaluation of targets and identification systems. The MTC distributions of novel SNPs were characterized in the both the Ghana collection and two other diverse collections of MTC strains (n = 175 in total).ResultsThe utility of various polymorphisms as species-, lineage-, and sublineage-defining phylogenetic markers for M. africanum was determined. Novel SNPs were also identified and found to be specific to either M. africanum West African-1 (Rv1332523; n = 32) or M. africanum West African-2 (nat751; n = 27). In the final analysis, a strain identification approach that combined multi-primer PCR targeting of the RD loci RD9, RD10, and RD702 was the most simple, straight-forward, and definitive means of distinguishing the two clades of M. africanum from one another and from other MTC species.ConclusionWith this study, we have organized a series of consistent phylogenetically-relevant markers for each of the distinct MTC lineages that share the M. africanum designation. A differential distribution of each M. africanum clade in Western Africa is described.

【 授权许可】

Unknown   
© Vasconcellos et al; licensee BioMed Central Ltd. 2010. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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