Real-time in vivo monitoring of magnetic nanoparticles in the bloodstream by AC biosusceptometry

Journal of Nanobiotechnology

Research

Gustavo M. Moretto¹  Ronaldo V. R. Matos¹  Ednaldo A. Zandoná¹  José R. A. Miranda¹  Caio C. Quini¹  Fábio P. F. Mello¹  André G. Próspero¹  Patrícia Fidelis-de-Oliveira¹  Marcos F. F. Calabresi¹  Nícholas Zufelato²  Andris F. Bakuzis³  Ricardo B. Oliveira⁴ 

[1] Biosciences Institute of Botucatu, São Paulo State University, Botucatu, São Paulo, Brazil;Physics Institute, Federal University of Goiás, Goiânia, Goiás, Brazil;Physics Institute, Federal University of Goiás, Goiânia, Goiás, Brazil;Instituto de Física-Universidade Federal de Goiás, 74690-900, Goiânia, GO, Brazil;Ribeirão Preto School of Medicine, São Paulo University, Ribeirão Prêto, São Paulo, Brazil;

关键词: AC biosusceptometry;    Magnetic nanoparticles;    Circulation time;    Pharmacokinetics;    Cardiovascular analysis;   

DOI  :  10.1186/s12951-017-0257-6

 received in 2016-11-16, accepted in 2017-03-10,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundWe introduce and demonstrate that the AC biosusceptometry (ACB) technique enables real-time monitoring of magnetic nanoparticles (MNPs) in the bloodstream. We present an ACB system as a simple, portable, versatile, non-invasive, and accessible tool to study pharmacokinetic parameters of MNPs, such as circulation time, in real time. We synthesized and monitored manganese doped iron oxide nanoparticles in the bloodstream of Wistar rats using two different injection protocols. Aiming towards a translational approach, we also simultaneously evaluated cardiovascular parameters, including mean arterial pressure, heart rate, and episodes of arrhythmia in order to secure the well-being of all animals.ResultsWe found that serial injections increased the circulation time compared with single injections. Immediately after each injection, we observed a transitory drop in arterial pressure, a small drop in heart rate, and no episodes of arrhythmia. Although some cardiovascular effects were observed, they were transitory and easily recovered in both protocols.ConclusionsThese results indicate that the ACB system may be a valuable tool for in vivo, real-time MNP monitoring that allows associations with other techniques, such as pulsatile arterial pressure and electrocardiogram recordings, helping ensuring the protocol safety, which is a fundamental step towards clinical applications.

【 授权许可】

CC BY   
© The Author(s) 2017