Spatial and seasonal influences on culturable endophytic mycobiota associated with different tissues of Eugenia jambolana Lam. and their antibacterial activity against MDR strains

BMC Microbiology

Research Article

Manila Yadav¹  Sandeep Kumar¹  Jaya Parkash Yadav¹  Amita Yadav¹ 

[1] Department of Genetics, M. D. University, 124001, Rohtak, Haryana, India;

关键词: Eugenia jambolana;    Season;    Sites;    Tissues;    Diversity;    Endophytic fungi;    Antibacterial;    MDR;   

DOI  :  10.1186/s12866-016-0664-0

 received in 2015-08-11, accepted in 2016-03-07,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundPresent study focuses on diversity and distribution analysis of endophytic fungi associated with different tissues of Eugenia jambolana. The influence of season and geographical location on diversity and distribution of endophytic fungi has been analyzed. Antibacterial activity of isolated fungal species has also been investigated against MDR bacterial strains.ResultA total of 1896 endophytic fungal isolates were obtained from healthy, surface sterilized tissues of leaf, stem and petiole tissues during summer, monsoon and winter season. Out of 24 fungal species isolated, 20 species belong to class Ascomycetes, 2 to Basidiomycetes and 2 to Zygomycetes. Maximum species diversity was in rainy season whereas colonization frequency was in winter. All the diversity indices showed maximum species diversity at site 5 (Yamunanager), rainy among the seasons and leaf among the tissues studied. Aspergillus genus was most frequently isolated. Aspergillus niger and Alternaria alternata were most dominant species. Three way ANOVA results showed that effect of season was highly significant on species diversity in relation to sites and tissues. 60 % endophytic fungal extracts showed significant antibacterial activity against one or more than one MDR bacterial strain.ConclusionDifferent fungal species were recovered from different sites but the inter-site comparisons were not significant according to Jaccard similarity coefficient. Diversity of such fungal endophytes indicates that Eugenia jambolana plant acts as an ecosystem facilitating survival of many microbes with impressive antibacterial potential.

【 授权许可】

CC BY   
© Yadav et al. 2016