Comparative genomics reveals Cyclospora cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens

BMC Genomics

Research Article

Longxian Zhang¹  Michael J. Arrowood²  Dawn M. Roellig²  Lihua Xiao²  Delynn M. Moss²  Michael A. Frace³  Kevin Tang³  Huajun Zheng⁴  Zhixiao Xu⁵  Yaoyu Feng⁵  Lin Wang⁵  Shiyou Liu⁶  Na Li⁶ 

[1] College of Animal Science and Veterinary Medicine, Henan Agricultural University, 450002, Zhengzhou, China;Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, 30333, Atlanta, GA, USA;Division of Scientific Resources, Centers for Disease Control and Prevention, 30333, Atlanta, GA, USA;Shanghai–Ministry of Science and Technology Key Laboratory of Health and Disease Genomics, Chinese National Human Genome Center at Shanghai, 250 Bibo Road, 201203, Shanghai, China;State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, 200237, Shanghai, China;State Key Laboratory of Bioreactor Engineering, School of Resources and Environmental Engineering, East China University of Science and Technology, 200237, Shanghai, China;Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, 30333, Atlanta, GA, USA;

关键词: Cyclospora;    Genomics;    Genome;    Genetics;    Evolution;    Apicomplexan;   

DOI  :  10.1186/s12864-016-2632-3

 received in 2015-12-10, accepted in 2016-04-20,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundCyclospora cayetanensis is an apicomplexan that causes diarrhea in humans. The investigation of foodborne outbreaks of cyclosporiasis has been hampered by a lack of genetic data and poor understanding of pathogen biology. In this study we sequenced the genome of C. cayetanensis and inferred its metabolism and invasion components based on comparative genomic analysis.ResultsThe genome organization, metabolic capabilities and potential invasion mechanism of C. cayetanensis are very similar to those of Eimeria tenella. Propanoyl-CoA degradation, GPI anchor biosynthesis, and N-glycosylation are some apparent metabolic differences between C. cayetanensis and E. tenella. Unlike Eimeria spp., there are no active LTR-retrotransposons identified in C. cayetanensis. The similar repertoire of host cell invasion-related proteins possessed by all coccidia suggests that C. cayetanensis has an invasion process similar to the one in T. gondii and E. tenella. However, the significant reduction in the number of identifiable rhoptry protein kinases, phosphatases and serine protease inhibitors indicates that monoxenous coccidia, especially C. cayetanensis, have limited capabilities or use a different system to regulate host cell nuclear activities. C. cayetanensis does not possess any cluster of genes encoding the TA4-type SAG surface antigens seen in E. tenella, and may use a different family of surface antigens in initial host cell interactions.ConclusionsOur findings indicate that C. cayetanensis possesses coccidia-like metabolism and invasion components but unique surface antigens. Amino acid metabolism and post-translation modifications of proteins are some major differences between C. cayetanensis and other apicomplexans. The whole genome sequence data of C. cayetanensis improve our understanding of the biology and evolution of this major foodborne pathogen and facilitate the development of intervention measures and advanced diagnostic tools.

【 授权许可】

CC BY   
© Liu et al. 2016