Quantitative imaging of mammalian transcriptional dynamics: from single cells to whole embryos

BMC Biology

Review

Valeria Levi¹  Stephanie Bissiere²  Melanie D. White²  Nicolas Plachta²  Ziqing W. Zhao² 

[1] Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires, C1428EHA, Conicet, Buenos Aires, Argentina;Institute of Molecular and Cell Biology, Agency for Science, Technology and Research (A*STAR), 61 Biopolis Drive, 138673, Singapore, Singapore;

关键词: Fluorescence Recovery After Photobleaching;    Fluorescence Correlation Spectroscopy;    Burst Size;    Target Search;    Nuclear Space;   

DOI  :  10.1186/s12915-016-0331-9

来源: Springer

【 摘 要 】

Probing dynamic processes occurring within the cell nucleus at the quantitative level has long been a challenge in mammalian biology. Advances in bio-imaging techniques over the past decade have enabled us to directly visualize nuclear processes in situ with unprecedented spatial and temporal resolution and single-molecule sensitivity. Here, using transcription as our primary focus, we survey recent imaging studies that specifically emphasize the quantitative understanding of nuclear dynamics in both time and space. These analyses not only inform on previously hidden physical parameters and mechanistic details, but also reveal a hierarchical organizational landscape for coordinating a wide range of transcriptional processes shared by mammalian systems of varying complexity, from single cells to whole embryos.

【 授权许可】

CC BY   
© Plachta et al. 2016