Methylation levels at IGF2 and GNAS DMRs in infants born to preeclamptic pregnancies

BMC Genomics

Research Article

Aiping Zhang¹  Rong Fang²  Hong Zhang³  Yun Liu⁴  Jiamei Ge⁵  Yuan Jiang⁶  Min Fang⁷  Jing He⁸  Danqing Yu⁸  Xiaoqun Ye⁸  Cong Han⁸  Minyue Dong⁹  Hefeng Huang⁹ 

[1] Bio-X Institutes, Key Laboratory for the Genetics of Developmental and Neuropsychiatric Disorders, Ministry of Education, Shanghai Jiao Tong University, Shanghai, China;Huzhou Maternity and Child Care Hospital, Huzhou, China;Institutes of Biomedical Sciences, Fudan University, Shanghai, China;Institutes of Biomedical Sciences, Fudan University, Shanghai, China;Department of Biochemistry and Molecular Biology, Key Laboratory of Molecular Medicine, the Ministry of Education, Fudan University Shanghai Medical College, Add: 303 Mingdao Building, 138 Yixueyuan Road, 200032, Shanghai, PR China;Jiaxing Maternity and Child Care Hospital, Jiaxing, China;Ningbo Women and Children’s Hospital, Ningbo, China;Shaoxing Women and Children’s Hospital, Shaoxing, China;Women’s Hospital, School of Medicine, Zhejiang University, Add: 1 Xueshi Road, 310006, Hangzhou, Zhejiang Province, China;Women’s Hospital, School of Medicine, Zhejiang University, Add: 1 Xueshi Road, 310006, Hangzhou, Zhejiang Province, China;Key Laboratory of Reproductive Genetics, Ministry of Education, Zhejiang University, Hangzhou, China;

关键词: Preeclampsia;    Offspring;    DNA methylation;    IGF2;    GNAS;    Metabolic diseases;   

DOI  :  10.1186/1471-2164-14-472

 received in 2013-03-04, accepted in 2013-07-09,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundOffspring of pregnancy complicated with preeclampsia are at high risk for hypertension, stroke and possibly obesity. The mechanisms behind the association of intrauterine exposure to preeclampsia and high risk of health problems in the later life remain largely unknown. The aims of the current investigation were to determine the changes in DNA methylation at IGF2 and GNAS DMR in offspring of preeclamptic pregnancy and to explore the possible mechanisms underlying the association between maternal preeclampsia and high risk for health problems in the later life of their offspring.ResultsUmbilical cord blood was taken from infants born to women of preeclampsia (n=56), gestational hypertension (n=23) and normal pregnancy (n=81). DNA methylation levels of IGF2 and GNAS DMR were determined by Massarray quantitative methylation analysis. Methylation levels at IGF2 DMR were significantly lower in preeclampsia than normal pregnancy. The average methylation level at IGF2 DMR was significantly correlated with preeclampsia even after birth weight, maternal age, gestational age at delivery and fetal gender were adjusted. The difference in methylation level was not significantly different between mild and severe preeclampsia. The methylation level at GNAS DMR was not significantly correlated with birth weight, maternal age, gestational age at delivery, fetal gender, preeclampsia or gestational hypertension.ConclusionsWe concluded preeclampsia induced a decrease in methylation level at IGF 2 DMR, and this might be among the mechanisms behind the association between intrauterine exposure to preeclampsia and high risk for metabolic diseases in the later life of the infants.

【 授权许可】

Unknown   
© He et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.