Metabolic profiling reveals altered sugar and secondary metabolism in response to UGPase overexpression in Populus

BMC Plant Biology

Research Article

Udaya C Kalluri¹  Gerald A Tuskan¹  Timothy J Tschaplinski¹  Sara S Jawdy¹  Raja S Payyavula¹  Robert W Sykes² 

[1] BioEnergy Science Center and Biosciences Division, Oak Ridge National Laboratory, 37831, Oak Ridge, TN, USA;The Biosciences Center, National Renewable Energy Laboratory, 80401, Golden, CO, USA;

关键词: Metabolic profiling;    Primary and secondary metabolism;    Cell wall;    UGPase;    Populus;   

DOI  :  10.1186/s12870-014-0265-8

 received in 2014-05-19, accepted in 2014-09-25,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundUDP-glucose pyrophosphorylase (UGPase) is a sugar-metabolizing enzyme (E.C. 2.7.7.9) that catalyzes a reversible reaction of UDP-glucose and pyrophosphate from glucose-1-phosphate and UTP. UDP-glucose is a key intermediate sugar that is channeled to multiple metabolic pathways. The functional role of UGPase in perennial woody plants is poorly understood.ResultsWe characterized the functional role of a UGPase gene in Populus deltoides, PdUGPase2. Overexpression of the native gene resulted in increased leaf area and leaf-to-shoot biomass ratio but decreased shoot and root growth. Metabolomic analyses showed that manipulation of PdUGPase2 results in perturbations in primary, as well as secondary metabolism, resulting in reduced sugar and starch levels and increased phenolics, such as caffeoyl and feruloyl conjugates. While cellulose and lignin levels in the cell walls were not significantly altered, the syringyl-to-guaiacyl ratio was significantly reduced.ConclusionsThese results demonstrate that PdUGPase2 plays a key role in the tightly coupled primary and secondary metabolic pathways and perturbation in its function results in pronounced effects on growth and metabolism beyond cell wall biosynthesis of Populus.

【 授权许可】

Unknown   
© Payyavula et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.