NMR Metabolic profiling of green tea (Camellia sinensis L.) leaves grown at Kemuning, Indonesia

International Conference on Science and Applied Science (Engineering and Educational Science) 2016

物理学;工业技术;教育

Wahyuni, D.S.C.^1,2 ; Kristanti, M.W.^1,2 ; Putri, R.K.^1,2 ; Rinanto, Y.^3

Pharmacy Department, Faculty of Mathematics and Sciences, Sebelas Maret University, Indonesia^1

Natural Medicine Research Group, Pharmacy Department, Faculty of Mathematics and Sciences, Sebelas Maret University, Indonesia^2

Biology Education Program, Faculty of Teacher Training and Education, Sebelas Maret University, Indonesia^3

关键词: Aspartic acids;    Camellia sinensis;    Medicinal plants;    Metabolic profiling;    Nuclear Magnetic Resonance (NMR);    Physical characteristics;    Secondary metabolites;    Succinic acids;   

Others  :  https://iopscience.iop.org/article/10.1088/1742-6596/795/1/012013/pdf
DOI  :  10.1088/1742-6596/795/1/012013

来源: IOP

【 摘 要 】

Green tea (Camellia sinensis L.) has been famous as a beverage and natural medicine. It contains a broad range of primary and secondary metabolites i.e. polyphenols. Nuclear Magnetic Resonance (NMR) has been widely used for metabolic profiling in medicinal plants. It provides a very fast and detailed analysis of the biomolecular composition of crude extracts. Moreover, an NMR spectrum is a physical characteristic of a compound and thus highly reproducible. Therefore, this study aims to profile metabolites of three different varieties of green tea C. Sinensis grown in Kemuning, Middle Java. Three varieties of green tea collected on Kemuning (TR1 2025, Gambung 4/5, and Chiaruan 143) were used in this study. 1H-NMR spectra were recorded at 230C on a 400 MHz Agilent WB (Widebore). The analysis was performed on dried green tea leaves and analyzed by 1H-NMR, 2D-J-resolved and 1H-1H correlated spectroscopy (COSY). MestRenova version 11.0.0 applied to identify metabolites in samples. A1H-NMR spectrum of tea showed amino acids and organic acids signal at the area δ 0.8-4.0. These were theanine, alanine, threonine, succinic acid, aspartic acid, lactic acid. Anomeric protons of carbohydrate were shown by the region of β-glucose, α-glucose, fructose and sucrose. The phenolic region was depicted at area δ 5.5-8.5. Epigallocatechin derivates and caffeine were detected in the tea leaves. The detail compound identification was observed and discussed in the text.