Evaluation of Mycobacterium tuberculosis viability in OMNIgene-SPUTUM reagent upon multi-day transport at ambient temperature

BMC Infectious Diseases

Technical Advance

Riccardo Alagna¹  Daniela Maria Cirillo¹  Elisa Tagliani¹  Hasan Hafizi²  Silva Tafaj² 

[1] Emerging Bacterial Pathogens Unit, Division of Immunology, Transplantation and Infectious Diseases, IRCCS San Raffaele Scientific Institute, Olgettina 58, 20132, Milan, Italy;University Hospital Shefqet Ndroqi, Tirana, Albania;

关键词: Mycobacterium tuberculosis;    Sample transport;    Liquid culture;    OMNIgene-SPUTUM;   

DOI  :  10.1186/s12879-017-2756-3

 received in 2017-05-24, accepted in 2017-09-21,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMaintaining the quality of clinical specimens for tuberculosis (TB) testing is a major challenge in many high TB burden-limited resources countries. Sample referral systems in low and middle income countries are often weak and the maintenance of the cold-chain challenging and very costly for TB programs. The development of transport media allowing the preservation of samples without refrigeration is critical for increasing access to TB diagnostic services and for reducing the costs related to testing.MethodsWe evaluated the performance of OMNIgene-SPUTUM (OM-S) reagent for the maintenance of Mycobacterium tuberculosis (MTB) viability in sputum samples in the absence of refrigeration and its capacity to stabilize nucleic acid for molecular testing. A total of 329 sputum specimens from presumptive TB cases collected at the National Reference Laboratory in Tirana, Albania, were either decontaminated by a conventional method or processed with OM-S reagent and stored at room temperature. Samples in OM-S were shipped to the Supranational Reference Laboratory in Milan, Italy, at various times and processed for liquid culture.ResultsOur data show that OM-S maintains MTB viability for at least three weeks in the absence of refrigeration and improves the quality of culture resulting in a contamination rate lower than 0.5%. However, a significant delay in the time to culture positivity was observed for samples stored for more than two weeks in OM-S.ConclusionsOverall, OM-S offers multiple benefits both at laboratory and TB national program level by increasing the availability to quality diagnostics, promoting access to health care services and strengthening TB patient care especially in hard to reach populations.

【 授权许可】

CC BY   
© The Author(s). 2017