Transcriptional analysis of the effect of exogenous decanoic acid stress on Streptomyces roseosporus

Microbial Cell Factories

Research

Qing Liu¹  Jianping Xie¹  Guojian Liao¹ 

[1] Institute of Modern Biopharmaceuticals, State Key Laboratory Breeding Base of Eco-Environment and Bio-Resource of the Three Gorges Area, School of life sciences, School of Pharmaceutical Sciences Southwest University,, 400715, Chongqing, China;

关键词: Daptomycin;    Decanoic acid;    Toxic;    Tolerance;    Streptomyces roseosporus;   

DOI  :  10.1186/1475-2859-12-19

 received in 2012-12-26, accepted in 2013-02-19,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroudDaptomycin is an important antibiotic against infections caused by drug-resistant pathogens. Its production critically depends on the addition of decanoic acid during fermentation. Unfortunately, decanoic acid (>2.5 mM) is toxic to daptomycin producer, Streptomyces roseosporus.ResultsTo understand the mechanism underlying decanoic tolerance or toxicity, the responses of S. roseosporus was determined by a combination of phospholipid fatty acid analysis, reactive oxygen species (ROS) measurement and RNA sequencing. Assays using fluorescent dyes indicated a sharp increase in reactive oxygen species during decanoic acid stress; fatty acid analysis revealed a marked increase in the composition of branched-chain fatty acids by approximately 10%, with a corresponding decrease in straight-chain fatty acids; functional analysis indicated decanoic acid stress has components common to other stress response, including perturbation of respiratory functions (nuo and cyd operons), oxidative stress, and heat shock. Interestingly, our transcriptomic analysis revealed that genes coding for components of proteasome and related to treholase synthesis were up-regulated in the decanoic acid –treated cells.ConclusionThese findings represent an important first step in understanding mechanism of decanoic acid toxicity and provide a basis for engineering microbial tolerance.

【 授权许可】

Unknown   
© Liao et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.