The Effects of Bee Venom on PLA2, COX-2, iNOS, AA and PG in RAW 264.7 Cells

Journal of Pharmacopuncture

Kee-Hyun Kim¹  Seong-No Lee¹  Seang-Jong Ha¹  Hyun-Chul Jo² 

[1] Department of Acupuncture & Moxibustion, College of Oriental Medicine, Kyung-won University;Department of Oriental Rehabilitation Medicine, College of Oriental Medicine, Kyung-won University;

关键词: Bee Venom;    Toxic;    PLA2;    COX-2;    iNOS;    AA;    PGD2;    PGE2;    RAW 264.7 Cell;   

DOI  :  10.3831/KPI.2002.5.2.040

来源: DOAJ

【 摘 要 】

Objectives : The purpose of this study was to investigate the effect of Bee Venom on the lipopolysaccharide-induced expression phospholipase A2, cyclooxygenase-2 and inducible nitrogen oxide synthase, and the generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells, a murine macrophage cell line. Methods : The expression of phospholipase A2 , cyclooxygenase and inducible nitrogen oxide synthase was determined by western blotting with corresponding antibodies, and the generation of arachidonic acid, prostaglandinD2 and E2 was assayed by ELISA method in RAW 264.7 cells. The non-toxic concentrations (0.1 to 5ug/ml) of bee venom determined by MTT assay, were used in this study. Results : 1. Bee venom inhibited lipopolysaccharide-induced expression of phospholipase A2in a dose dependent manner after 48 hours treatment. 2. Bee venom inhibited lipopolysaccharide-induced expression of cyclooxygenase-2 in a dose dependent manner after 24 and 48 hours treatment. 3. Bee venom inhibited lipopolysaccharide-induced expression of inducible nitrogen oxidesynthase in a dose dependent manner after 48 hours treatment. 4. The generation of arachidonic acid, prostaglandin D2 and E2was not much affected by the treatment of bee venom on the lipopolysaccharide-induced generation of arachidonic acid, prostaglandin D2 and E2 in RAW 264.7 cells.

【 授权许可】

Unknown