Clinical implications of AGBL2 expression and its inhibitor latexin in breast cancer

World Journal of Surgical Oncology

Research

Caigang Liu¹  Hao Zhang¹  Yuan Ren²  Deyan Pang³ 

[1] Department of Breast Surgery, Second hospital of Dalian Medical University, Zhongshan Road, 116023, Dalian, People’s Republic of China;Department of Hematology, First hospital of China Medical University, Nanjing Street, 110001, Shenyang, People’s Republic of China;Department of Mathematics, Northeast Yucai School, Shiji Road, 110179, Shenyang, People’s Republic of China;

关键词: Breast cancer;    Cancer stem cell;    AGBL2;    latexin;    Survival;   

DOI  :  10.1186/1477-7819-12-142

 received in 2013-08-31, accepted in 2014-04-20,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundWe investigated the expression status of AGBL2 and its inhibitor latexin in breast cancer stem cells and its clinical implications in order to lay a foundation for managing breast cancer.MethodsCD44+/CD24- tumor cells (CSC) from clinical specimens were sorted using flow cytometry. AGBL2 expression status was detected in CSC and 126 breast cancer specimens by western blot and immunohistochemistry staining. The relationship between the AGBL2 protein and clinicopathological parameters and prognosis was subsequently determined.ResultAs a result, CSC are more likely to generate new tumors in mice and cell microspheres that are deficient in non-obese diabetic/severe combined immunodeficiency mice (NOD/SCID) compared to the control group. The AGBL2 protein was expressed higher in CSC induced to epithelial to mesenchymal transition (EMT) when compared to the control cells, and was found to be related to CSC chemotherapy resistance. After Spearman regression correlation analysis, AGBL2 was observed to be related to clinical stage, histological stage, and lymph node metastasis. In the Cox regression test, the AGBL2 protein was detected as an independent prognostic factor. Through immunoprecipitation, AGBL2 and latexin could form immune complexes.ConclusionsThese results demonstrate that AGBL2 is a latexin- interacting protein that regulates the tubulin tyrosination cycle and is a potential target for intervention.

【 授权许可】

Unknown   
© Zhang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.