Aluminum overload increases oxidative stress in four functional brain areas of neonatal rats

Journal of Biomedical Science

Research

Guoo-Shyng Wang Hsu¹  Chia-Yi Yuan²  Yih-Jing Lee³ 

[1] Department of Nutritional Science, Fu-Jen Catholic University, 510 Chung-Cheng Road, 24205, New Taipei City, Hsinchuang, Taiwan;Graduate Institute of Nutrition and Food Sciences, Fu-Jen Catholic University, New Taipei City, Hsinchuang, Taiwan;School of Medicine, Fu-Jen Catholic University, 510 Chung-Cheng Road, 24205, New Taipei City, Hsinchuang, Taiwan;

关键词: Aluminum;    Neonatal rats;    Functional brain tissues;    Intraperitoneal injection;   

DOI  :  10.1186/1423-0127-19-51

 received in 2012-03-11, accepted in 2012-05-21,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundHigher aluminum (Al) content in infant formula and its effects on neonatal brain development are a cause for concern. This study aimed to evaluate the distribution and concentration of Al in neonatal rat brain following Al treatment, and oxidative stress in brain tissues induced by Al overload.MethodsPostnatal day 3 (PND 3) rat pups (n =46) received intraperitoneal injection of aluminum chloride (AlCl3), at dosages of 0, 7, and 35 mg/kg body wt (control, low Al (LA), and high Al (HA), respectively), over 14 d.ResultsAluminum concentrations were significantly higher in the hippocampus (751.0 ± 225.8 ng/g v.s. 294.9 ± 180.8 ng/g; p < 0.05), diencephalon (79.6 ± 20.7 ng/g v.s. 20.4 ± 9.6 ng/g; p < 0.05), and cerebellum (144.8 ± 36.2 ng/g v.s. 83.1 ± 15.2 ng/g; p < 0.05) in the HA group compared to the control. The hippocampus, diencephalon, cerebellum, and brain stem of HA animals displayed significantly higher levels of lipid peroxidative products (TBARS) than the same regions in the controls. However, the average superoxide dismutase (SOD) activities in the cerebral cortex, hippocampus, cerebellum, and brain stem were lower in the HA group compared to the control. The HA animals demonstrated increased catalase activity in the diencephalon, and increased glutathione peroxidase (GPx) activity in the cerebral cortex, hippocampus, cerebellum, and brain stem, compared to controls.ConclusionAluminum overload increases oxidative stress (H2O2) in the hippocampus, diencephalon, cerebellum, and brain stem in neonatal rats.

【 授权许可】

Unknown   
© Yuan et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.