Comparison of the specificity of antibodies to VAR2CSA in Cameroonian multigravidae with and without placental malaria: a retrospective case–control study

Malaria Journal

Research

Ali Salanti¹  Diane W. Taylor²  John J. Chen²  Andrew Wey²  Rui Fang²  Anna Babakhanyan²  Canisia Efundem³  Rose G. F. Leke³  Robert J. I. Leke³  Grace Sama³ 

[1] Department of Immunology and Microbiology, Centre for Medical Parasitology, University of Copenhagen, Copenhagen, Denmark;Department of Tropical Medicine, Medical Microbiology and Pharmacology, John A Burns School of Medicine, University of Hawaii at Manoa, 651 Ilalo Street, BSB 320, 96813, Honolulu, HI, USA;Faculty of Medicine and Biomedical Research, Biotechnology Centre, University of Yaounde 1, Yaounde, Cameroon;

关键词: Antibodies;    Avidity;    VAR2CSA;    Placental malaria;   

DOI  :  10.1186/s12936-015-1023-6

 received in 2015-09-22, accepted in 2015-11-26,  发布年份 2015

来源: Springer

【 摘 要 】

BackgroundAntibodies (Ab) to VAR2CSA prevent Plasmodium falciparum-infected erythrocytes from sequestrating in the placenta, i.e., prevent placental malaria (PM). The specificity of Ab to VAR2CSA associated with absence of PM is unknown. Accordingly, differences in the specificity of Ab to VAR2CSA were compared between multigravidae with and without PM who had Ab to VAR2CSA.MethodsIn a retrospective case–control study, plasma collected from Cameroonian multigravidae with (n = 96) and without (n = 324) PM were screened in 21 assays that measured antibody levels to full length VAR2CSA (FV2), individual VAR2CSA DBL domains, VAR2CSA domains from different genetic backgrounds (variants), as well as proportion of high avidity Ab to FV2.ResultsMultigravidae with and without PM had similar levels of Ab to FV2, the six VAR2CSA DBL domains and different variants, while the proportion of high avidity Ab to FV2 was significantly higher in women without PM at delivery (p = 0.0030) compared to women with PM. In a logistic regression model adjusted for gravidity and age, the percentage of high avidity Ab to FV2 was associated with reduced likelihood of PM in multigravidae. A 5 % increase in proportion of high avidity Ab to FV2 was associated with a nearly 15 % lower likelihood of PM.ConclusionAb avidity to FV2 may be an important indicator of immunity to PM.

【 授权许可】

CC BY   
© Babakhanyan et al. 2015