Development of bio-based fine chemicalproduction through synthetic bioengineering

Microbial Cell Factories

Review

Akihiko Kondo¹  Satoshi Wakai²  Tomohisa Hasunuma²  Kiyotaka Y Hara²  Michihiro Araki²  Naoko Okai² 

[1] Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada, 657-8501, Kobe, Japan;Organization of Advanced Science and Technology, Kobe University, Nada, Kobe, Japan;

关键词: Fine chemical;    Synthetic bioengineering;    Metabolic engineering;    Enzymatic synthesis;    Microbial fermentation;    Bioinformatics;   

DOI  :  10.1186/s12934-014-0173-5

 received in 2014-07-31, accepted in 2014-11-23,  发布年份 2014

来源: Springer

【 摘 要 】

Fine chemicals that are physiologically active, such as pharmaceuticals,cosmetics, nutritional supplements, flavoring agents as well as additives for foods,feed, and fertilizer are produced by enzymatically or through microbialfermentation. The identification of enzymes that catalyze the target reaction makespossible the enzymatic synthesis of the desired fine chemical. The genes encodingthese enzymes are then introduced into suitable microbial hosts that are culturedwith inexpensive, naturally abundant carbon sources, and other nutrients. Metabolicengineering create efficient microbial cell factories for producing chemicals athigher yields. Molecular genetic techniques are then used to optimize metabolicpathways of genetically and metabolically well-characterized hosts. Syntheticbioengineering represents a novel approach to employ a combination of computersimulation and metabolic analysis to design artificial metabolic pathways suitablefor mass production of target chemicals in host strains. In the present review, wesummarize recent studies on bio-based fine chemical production and assess thepotential of synthetic bioengineering for further improving theirproductivity.

【 授权许可】

Unknown   
© Hara et al.; licensee BioMed Central. 2014. This is an Open Access article distributed under the terms of theCreative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproductionin any medium, provided the original work is properly credited. The CreativeCommons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unlessotherwise stated.