ATP regulation inbioproduction

Microbial Cell Factories

Review

Akihiko Kondo¹  Kiyotaka Y. Hara² 

[1] Department of Chemical Science and Engineering, Graduate School of Engineering, Kobe University, 1-1 Rokkodaicho, Nada-ku, 657-8501, Kobe, Japan;Department of Environmental Sciences, Graduate School of Nutritional and Environmental Sciences, University of Shizuoka, 52-1 Yada, Suruga-ku, 422-8526, Shizuoka, Japan;

关键词: ATP;    Bioproduction;    Energy metabolism;    Metabolic engineering;    Synthetic bioengineering;   

DOI  :  10.1186/s12934-015-0390-6

 received in 2015-09-14, accepted in 2015-11-25,  发布年份 2015

来源: Springer

【 摘 要 】

Adenosine-5′-triphosphate (ATP) is consumed as a biological energysource by many intracellular reactions. Thus, the intracellular ATP supply isrequired to maintain cellular homeostasis. The dependence on the intracellular ATPsupply is a critical factor in bioproduction by cell factories. Recent studies haveshown that changing the ATP supply is critical for improving product yields. In thisreview, we summarize the recent challenges faced by researchers engaged in thedevelopment of engineered cell factories, including the maintenance of a large ATPsupply and the production of cell factories. The strategies used to enhance ATPsupply are categorized as follows: addition of energy substrates, controlling pH,metabolic engineering of ATP-generating or ATP-consuming pathways, and controllingreactions of the respiratory chain. An enhanced ATP supply generated using thesestrategies improves target production through increases in resource uptake, cellgrowth, biosynthesis, export of products, and tolerance to toxic compounds.

【 授权许可】

CC BY   
© Hara and Kondo. 2015