Characterization and functional analysis of the Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) gene family in poplar

PeerJ

article

Nan Chao¹  Qi Qi²  Shuang Li²  Brent Ruan⁵  Xiangning Jiang²  Ying Gai² 

[1] School of Life Science, Tsinghua University;College of Biological Sciences and Biotechnology, Beijing Forestry University;School of Biotechnology, Jiangsu University of Science and Technology;College of Horticulture, China Agricultural University;Department of Agricultural and Biological Engineering, University of Illinois at Urbana Champaign;National Engineering Laboratory for Tree Breeding, the Tree and Ornamental Plant Breeding and Biotechnology Laboratory of Chinese Forestry Administration

关键词: Hydroxycinnamoyl- CoA: shikimate hydroxycinnamoyl transferase;    Enzymatic synthesis;    Divergence;    Gene family;    Monolignol;    Populus;   

DOI  :  10.7717/peerj.10741

学科分类：社会科学、人文和艺术（综合）

来源: Inra

【 摘 要 】

Hydroxycinnamoyl-CoA: shikimate hydroxycinnamoyl transferase (HCT) divides the mass flux to H, G and S units in monolignol biosynthesis and affects lignin content. Ten HCT homologs were identified in the Populus trichocarpa (Torr. & Gray) genome. Both genome duplication and tandem duplication resulted in the expansion of HCT orthologs in Populus. Comprehensive analysis including motif analysis, phylogenetic analysis, expression profiles and co-expression analysis revealed the divergence and putative function of these candidate PoptrHCTs. PoptrHCT1 and 2 were identified as likely involved in lignin biosynthesis. PoptrHCT9 and 10- are likely to be involved in plant development and the response to cold stress. Similar functional divergence was also identified in Populus tomentosa Carr. Enzymatic assay of PtoHCT1 showed that PtoHCT1 was able to synthesize caffeoyl shikimate using caffeoyl-CoA and shikimic acid as substrates.

【 授权许可】

CC BY