First imported relapse case of Plasmodium vivax malaria and analysis of its origin by CSP sequencing in Henan Province, China

Malaria Journal

Case Report

Cheng-yun Yang¹  Rui-min Zhou¹  Dan Qian¹  Yu-ling Zhao¹  Bian-li Xu¹  Ying Liu¹  Hong-wei Zhang¹ 

[1] Henan Center for Disease Control and Prevention, 450016, Zhengzhou, China;

关键词: Plasmodium vivax;    Relapse;    Imported;    CSP;   

DOI  :  10.1186/1475-2875-13-448

 received in 2014-06-30, accepted in 2014-11-10,  发布年份 2014

来源: Springer

【 摘 要 】

In recent years, there has been a substantial increase of imported Plasmodium vivax incidence in Henan Province. As China is in a pre-elimination phase, the surveillance of imported malaria is essential, but there is no good way to distinguish imported cases from indigenous cases. This paper reports a case of a 39-year-old man who acquired P. vivax while staying in Indonesia for one month in 2013, and relapsed in Henan, China in 2014. This was diagnosed as vivax malaria based on rapid diagnostic test, Giemsa-stained peripheral blood smear and Plasmodium species-specific nested PCR. The genetic sequence for the circumsporozoite protein genes was analysed and the genetic variations were compared with a previously constructed database of Chinese isolates. The results from the circumsporozoite protein (CSP) gene sequence analysis centered on the repeat patterns showed that the imported cases had completely different sequences from any subtypes from Chinese isolates, but well matched with the countries travelled by the patient. The imported vivax cases were able to clearly distinguish from the indigenous vivax cases by detecting the CSP gene and were able to confim its origin by genotyping.

【 授权许可】

Unknown   
© Liu et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.