Comparison of endometrial regenerative cells and bone marrow stromal cells

Journal of Translational Medicine

Research

Marianna Sabatino¹  Jiaqiang Ren¹  Ping Jin¹  Sara Civini¹  Huan Wang¹  David F Stroncek²  Vladimir Bogin³  Thomas E Ichim³ 

[1] Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA;Department of Transfusion Medicine, Clinical Center, National Institutes of Health, Bethesda, MD, USA;Department of Transfusion Medicine, Cell Processing Section, 10 Center Drive-MSC-1288, Building 10, Room 3C720, 20892-1288, Bethesda, MD, USA;MediStem Inc, San Diego, CA, USA;

关键词: Endometrial regenerative cells;    Bone marrow stromal cells;    Mesenchymal stromal cells;    Mesenchymal stem cells;    Regenerative medicine;   

DOI  :  10.1186/1479-5876-10-207

 received in 2012-07-31, accepted in 2012-09-19,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundEndometrial regenerative cells (ERC) and bone marrow stromal cells (BMSC) are being used in clinical trials. While they have been reported to have similar characteristics, they have not been directly compared.MethodsWe compared micro RNA (miRNA) and gene expression profiles, soluble cytokine and growth factor levels and ability to inhibit ongoing mixed leukocyte reaction (MLR) of ERC and BMSC each derived from 6 healthy subjects.ResultsERC and BMSC miRNA and gene expression profiles were similar, but not identical; more differences were noted in the expression of genes than in miRNAs. Genes overexpressed in ERCs were more likely to be in immune and inflammation pathways and those overexpressed in BMSCs were more likely to be in stem cell and cancer signaling pathways. In addition, the levels of IL-8 and ICAM-1 were greater in ERC supernatants while the levels of HGF, VEGF, IL-6, CXCL12, TGFB1 and TGFB2 were greater in BMSC supernatants. Additionally, ERC demonstrated greater inhibition of the proliferation of mixed leukocyte cultures.ConclusionsThese results suggest that the in vivo effects of ERC and BMSC may differ. Multiple properties of stromal cells are responsible for their in vivo effectiveness and ERC may be more effective for some of the clinical applications and BMSC for others. Studies in animal models or clinical trials will be required to more fully characterize the differences between ERC and BMSC.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.