Variability of antibiotic susceptibility and toxin production of Staphylococcus aureus strains isolated from skin, soft tissue, and bone related infections

BMC Microbiology

Research Article

Simeon O Kotchoni¹  Ymkje Stienstra²  Théodora A Ahoyo³  Honoré S Bankolé⁴  Haziz Sina⁵  Lamine Baba-Moussa⁵  Yves Barogui⁶  Daniel Keller⁷  Gilles Prévost⁷  Wardi Moussaoui⁷ 

[1] Department of Biology and Center for Computational and Integrative Biology, Rutgers University, 08102, Camden, NJ, USA;Department of Internal Medicine/Infectious Diseases, University of Groningen, University Medical Center Groningen, P.O. Box 30.001, 9700 RB, Groningen, The Netherlands;EPAC/ Université d’Abomey-Calavi, 01 BP 526, Cotonou, BENIN;Laboratoire National de Santé Publique, Cotonou, Bénin;Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Faculté des Sciences et Techniques/Université d’Abomey-Calavi, 05 BP 1604, Cotonou, BENIN;Programme National de Lutte contre l'Ulcère de Buruli, Cotonou, Benin;Unité: EA-4438 Physiopathologie et Médecine, Institut de Bactériologie, 3 rue Koeberlé, F-67000, Strasbourg, France;

关键词: S. aureus;    MRSA;    PVL;    Pyomyositis;    Osteomyelitis;    Skin infections;    Benin;    Africa;   

DOI  :  10.1186/1471-2180-13-188

 received in 2013-03-20, accepted in 2013-08-07,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundStaphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic resistance of S. aureus strains isolated from skin, soft tissue, and bone related infections.ResultsA total of 136 S. aureus strains were collected from five different types of infection: furuncles, pyomyositis, abscesses, Buruli ulcers, and osteomyelitis, from hospital admissions and out-patients in Benin. All strains were resistant to benzyl penicillin, while 25% were resistant to methicillin, and all showed sensitivity to vancomycin. Panton-Valentine leukocidin (PVL) was the most commonly produced virulence factor (70%), followed by staphylococcal enterotoxin B (44%). Exfoliative toxin B was produced by 1.3% of the strains, and was only found in isolates from Buruli ulcers. The tsst-1, sec, and seh genes were rarely detected (≤1%).ConclusionsThis study provides new insight into the prevalence of toxin and antibiotic resistance genes in S. aureus strains responsible for skin, soft tissue, and bone infections. Our results showed that PVL was strongly associated with pyomyositis and osteomyelitis, and that there is a high prevalence of PVL-MRSA skin infections in Benin.

【 授权许可】

Unknown   
© Sina et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.