Variability of antibiotic susceptibility and toxin production of Staphylococcus aureus strains isolated from skin, soft tissue, and bone related infections

BMC Microbiology

Lamine Baba-Moussa⁷  Gilles Prévost³  Simeon O Kotchoni²  Ymkje Stienstra¹  Yves Barogui⁶  Honoré S Bankolé⁴  Daniel Keller³  Wardi Moussaoui³  Théodora A Ahoyo⁵  Haziz Sina⁷ 

[1]Department of Internal Medicine/Infectious Diseases, University of Groningen, University Medical Center Groningen, P.O. Box 30.001, Groningen 9700 RB, The Netherlands

[2]Department of Biology and Center for Computational and Integrative Biology, Rutgers University, Camden NJ 08102, USA

[3]Unité: EA-4438 Physiopathologie et Médecine, Institut de Bactériologie, 3 rue Koeberlé, Strasbourg F-67000, France

[4]Laboratoire National de Santé Publique, Cotonou, Bénin

[5]EPAC/ Université d’Abomey-Calavi, Cotonou 01 BP 526, BENIN

[6]Programme National de Lutte contre l'Ulcère de Buruli, Cotonou, Benin

[7]Laboratoire de Biologie et de Typage Moléculaire en Microbiologie

[8] Faculté des Sciences et Techniques/Université d’Abomey-Calavi, Cotonou 05 BP 1604, BENIN

关键词: Africa;    Benin;    Skin infections;    Osteomyelitis;    Pyomyositis;    PVL;    MRSA;    S. aureus;   

Others  :  1143273
DOI  :  10.1186/1471-2180-13-188

 received in 2013-03-20, accepted in 2013-08-07,  发布年份 2013

【 摘 要 】

Background

Staphylococcus aureus is an opportunistic commensal bacterium that mostly colonizes the skin and soft tissues. The pathogenicity of S. aureus is due to both its ability to resist antibiotics, and the production of toxins. Here, we characterize a group of genes responsible for toxin production and antibiotic resistance of S. aureus strains isolated from skin, soft tissue, and bone related infections.

Results

A total of 136 S. aureus strains were collected from five different types of infection: furuncles, pyomyositis, abscesses, Buruli ulcers, and osteomyelitis, from hospital admissions and out-patients in Benin. All strains were resistant to benzyl penicillin, while 25% were resistant to methicillin, and all showed sensitivity to vancomycin. Panton-Valentine leukocidin (PVL) was the most commonly produced virulence factor (70%), followed by staphylococcal enterotoxin B (44%). Exfoliative toxin B was produced by 1.3% of the strains, and was only found in isolates from Buruli ulcers. The tsst-1, sec, and seh genes were rarely detected (≤1%).

Conclusions

This study provides new insight into the prevalence of toxin and antibiotic resistance genes in S. aureus strains responsible for skin, soft tissue, and bone infections. Our results showed that PVL was strongly associated with pyomyositis and osteomyelitis, and that there is a high prevalence of PVL-MRSA skin infections in Benin.

【 授权许可】

   
2013 Sina et al.; licensee BioMed Central Ltd.

【 图 表 】

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