Isolation And Partial Characterization Of Bacteria Activity Associated With Gorgonian Euplexaura sp. Against Methicillin-Resistant Staphylococcus aureus (MRSA)

2nd International Conference on Tropical and Coastal Region Eco Development 2016

地球科学;生态环境科学

Kristiana, R.^1 ; Ayuningrum, D.^1 ; Asagabaldan, M.A.^1 ; Nuryadi, H.^1 ; Sabdono, A.^2 ; Radjasa, O.K.^2,3 ; Trianto, A.^2

Coastal Resources Management, Faculty of Fisheries and Marine Sciences, Diponegoro University, Tembalang, Semarang

50275, Indonesia^1

Department of Marine Sciences, Faculty of Fisheries and Marine Sciences, Diponegoro University, Tembalang, Semarang

50275, Indonesia^2

Ministry of Research, Technology and Higher Education of the Republic of Indonesia, 10270, Indonesia^3

关键词: Anti-bacterial activity;    antibacterial;    Euplexaura sp;    Methicillin-resistant staphylococcus aureus;    Molecular identification;    MRSA;    Partial characterization;    Staphylococcus aureus;   

Others  :  https://iopscience.iop.org/article/10.1088/1755-1315/55/1/012056/pdf
DOI  :  10.1088/1755-1315/55/1/012056

学科分类：环境科学（综合）

来源: IOP

【 摘 要 】

Methicillin-resistant Staphylococcus aureus (MRSA) infection has emerged in around the world and has been resistance to ciprofloxacin, erythromycin, clindamycin. The aims of this study were to isolate, to investigate and to characterize bacterial symbionts gorgonian having activity against MRSA. Euplexaura sp. was collected from Panjang Island, Jepara, Indonesia by snorkling 2-5 m in depth. Bacterias were isolated by using spesific media with dilution method. Bacterias were conducted by using the streak method. Antibacterial activity was investigated by overlay method. The potent bacteria was identified by using molecular identification (DNA extraction, electrophoresis, PCR and phylogenetic analysis using 16S rDNA genes with actinobacteria-spesific primers) and bio-chemical test (among 5 isolated bacteria from gorgonian showed activity against MRSA). The strain PG-344 was the best candidat that has an inhibition zone against MRSA. The result of sequencing bacteria is 100% closely related with Virgibacillus salarius. This becomes a potential new bioactive compounds to against MRSA that can be a new drug discovery.