Identification of candidate genes involved in Witches’ broom disease resistance in a segregating mapping population of Theobroma cacao L. in Brazil

BMC Genomics

Research Article

Johannes Jansen¹  Daniela Viana da Silva²  Ronan Xavier Corrêa²  Samuel Martins de Jesus Branco²  Ioná Santos Araújo³  Stefan Royaert⁴  Jean-Philippe Marelli⁴  Donald S. Livingstone⁵  Juan Carlos Motamayor⁵  Guiliana Mustiga⁵ 

[1] Biometris, Wageningen University and Research Centre, P.O. Box 100, 6700 AC, Wageningen, The Netherlands;Departamento de Ciências Biológicas, Universidade Estadual de Santa Cruz, Rodovia Ilhéus-Itabuna, Km 16, Bairro Salobrinho, CEP 45.662-900, Ilhéus, BA, Brazil;Departamento de Ciências Vegetais, Universidade Federal Rural do Semi-Arido, BR 110 - Km 47, Bairro Pres. Costa e Silva, CEP 59.625-900, Mossoró, RN, Brazil;Mars Center for Cocoa Science, CP 55, CEP 45.630-000, Itajuípe, BA, Brazil;Mars, Incorporated, 13601 Old Cutler Road, 33158, Miami, FL, USA;

关键词: Theobroma cacao;    SNP;    Genetic linkage map;    Witches’ broom disease;    Marker-trait associations;    Candidate genes;   

DOI  :  10.1186/s12864-016-2415-x

 received in 2015-05-14, accepted in 2016-01-26,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundWitches’ broom disease (WBD) caused by the fungus Moniliophthora perniciosa is responsible for considerable economic losses for cacao producers. One of the ways to combat WBD is to plant resistant cultivars. Resistance may be governed by a few genetic factors, mainly found in wild germplasm.ResultsWe developed a dense genetic linkage map with a length of 852.8 cM that contains 3,526 SNPs and is based on the MP01 mapping population, which counts 459 trees from a cross between the resistant ‘TSH 1188’ and the tolerant ‘CCN 51’ at the Mars Center for Cocoa Science in Barro Preto, Bahia, Brazil. Seven quantitative trait loci (QTL) that are associated with WBD were identified on five different chromosomes using a multi-trait QTL analysis for outbreeders. Phasing of the haplotypes at the major QTL region on chromosome IX on a diversity panel of genotypes clearly indicates that the major resistance locus comes from a well-known source of WBD resistance, the clone ‘SCAVINA 6’. Various potential candidate genes identified within all QTL may be involved in different steps leading to disease resistance. Preliminary expression data indicate that at least three of these candidate genes may play a role during the first 12 h after infection, with clear differences between ‘CCN 51’ and ‘TSH 1188’.ConclusionsWe combined the information from a large mapping population with very distinct parents that segregate for WBD, a dense set of mapped markers, rigorous phenotyping capabilities and the availability of a sequenced genome to identify several genomic regions that are involved in WBD resistance. We also identified a novel source of resistance that most likely comes from the ‘CCN 51’ parent. Thanks to the large population size of the MP01 population, we were able to pick up QTL and markers with relatively small effects that can contribute to the creation and selection of more tolerant/resistant plant material.

【 授权许可】

CC BY   
© Royaert et al. 2016