Construction and analysis of a high-density genetic linkage map in cabbage (Brassica oleracea L. var. capitata)

BMC Genomics

Research Article

Wei Hua¹  Shunmou Huang¹  Shengyi Liu¹  Jifeng Sun²  Yangyong Zhang²  Zhiyuan Fang²  Suxia Yuan²  Wanxing Wang²  Aisong Zeng²  Yumei Liu²  Limei Yang²  Mu Zhuang² 

[1] Institute of Oil Crops Research, Chinese Academy of Agriculture Sciences, Key Laboratory for Biological Sciences of Oil Crops, P.R.China. Ministry of Agriculture, 430062, Wuhan, China;Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Key Laboratory of Biology and Genetic Improvement of Horticultural Crops, Ministry of Agriculture, P.R.China, 100081, Beijing, China;

关键词: Cabbage;    Brassica;    Genetic linkage map;    SSR;    SNP;    Genome;   

DOI  :  10.1186/1471-2164-13-523

 received in 2012-03-01, accepted in 2012-09-18,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundBrassica oleracea encompass a family of vegetables and cabbage that are among the most widely cultivated crops. In 2009, the B. oleracea Genome Sequencing Project was launched using next generation sequencing technology. None of the available maps were detailed enough to anchor the sequence scaffolds for the Genome Sequencing Project. This report describes the development of a large number of SSR and SNP markers from the whole genome shotgun sequence data of B. oleracea, and the construction of a high-density genetic linkage map using a double haploid mapping population.ResultsThe B. oleracea high-density genetic linkage map that was constructed includes 1,227 markers in nine linkage groups spanning a total of 1197.9 cM with an average of 0.98 cM between adjacent loci. There were 602 SSR markers and 625 SNP markers on the map. The chromosome with the highest number of markers (186) was C03, and the chromosome with smallest number of markers (99) was C09.ConclusionsThis first high-density map allowed the assembled scaffolds to be anchored to pseudochromosomes. The map also provides useful information for positional cloning, molecular breeding, and integration of information of genes and traits in B. oleracea. All the markers on the map will be transferable and could be used for the construction of other genetic maps.

【 授权许可】

Unknown   
© Wang et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.