Identification of effector-like proteins in Trichoderma spp. and role of a hydrophobin in the plant-fungus interaction and mycoparasitism

BMC Genetics

Research Article

Paulina Guzmán-Guzmán¹  Vianey Olmedo-Monfil¹  Alfredo Herrera-Estrella²  Mario Iván Alemán-Duarte³  Luis Delaye⁴ 

[1] División de Ciencias Naturales y Exactas, Departamento de Biología, Universidad de Guanajuato, Guanajuato, Gto, Mexico;Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico;Laboratorio Nacional de Genómica para la Biodiversidad, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico;Unidad Irapuato, Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico;Unidad Irapuato, Irapuato, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Gto, Mexico;

关键词: Trichoderma;    Effector;    Plant-fungus interaction;    Mycoparasitism;    Hydrophobin;   

DOI  :  10.1186/s12863-017-0481-y

 received in 2016-10-02, accepted in 2017-02-07,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundTrichoderma spp. can establish beneficial interactions with plants by promoting plant growth and defense systems, as well as, antagonizing fungal phytopathogens in mycoparasitic interactions. Such interactions depend on signal exchange between both participants and can be mediated by effector proteins that alter the host cell structure and function, allowing the establishment of the relationship. The main purpose of this work was to identify, using computational methods, candidates of effector proteins from T. virens, T. atroviride and T. reesei, validate the expression of some of the genes during a beneficial interaction and mycoparasitism and to define the biological function for one of them.ResultsWe defined a catalogue of putative effector proteins from T. virens, T. atroviride and T. reesei. We further validated the expression of 16 genes encoding putative effector proteins from T. virens and T. atroviride during the interaction with the plant Arabidopsis thaliana, and with two anastomosis groups of the phytopathogenic fungus Rhizoctonia solani. We found genes which transcript levels are modified in response to the presence of both plant fungi, as well as genes that respond only to either a plant or a fungal host. Further, we show that overexpression of the gene tvhydii1, a Class II hydrophobin family member, enhances the antagonistic activity of T. virens against R. solani AG2. Further, deletion of tvhydii1 results in reduced colonization of plant roots, while its overexpression increases it.ConclusionsOur results show that Trichoderma is able to respond in different ways to the presence of a plant or a fungal host, and it can even distinguish between different strains of fungi of a given species. The putative effector proteins identified here may play roles in preventing perception of the fungus by its hosts, favoring host colonization or protecting it from the host’s defense response. Finally, the novel effector protein TVHYDII1 plays a role in plant root colonization by T, virens, and participates in its antagonistic activity against R. solani.

【 授权许可】

CC BY   
© The Author(s). 2017