Genetic diversity of Plasmodium vivax population in Anhui province of China

Malaria Journal

Research

Yucheng Xu¹  Fei Xu¹  Fangli Lu²  Bo Huang²  Xuchu Hu²  Shanqing Wang³  Xin-zhuan Su⁴  Hong Guo⁵  Shiguang Huang⁶  Yaming Yang⁷ 

[1] Clinical Laboratory, Licang Town Hospital of Mengcheng County, 233500, Anhui province, China;Department of Parasitology, Zhongshan School of Medicine, Sun Yat-sen University, 510080, Guangzhou, Guangdong, China;Ministry of Education, Key Laboratory of Tropical Disease Control (Sun Yat-sen University), 510080, Guangzhou, Guangdong, China;Hainan CDC, 570203, Haikou, Hainan, China;Laboratory of Malaria and Vector Research, National Institute of Allergy and Infectious Diseases, National Institutes of Health, 20892, Bethesda, MD, USA;State Key Laboratory of Cellular Stress Biology, School of Life Science, Xiamen University, 361005, Xiamen, Fujian, China;Public Laboratory of Hainan Medical University, 3 Xueyuan Road, 571199, Haikou, Hainan, China;School of Medicine, Jinan University, 510632, Guangzhou, China;Yunnan Institute of Parasitic Diseases, 665000, Puer, Yunnan, China;

关键词: Plasmodium vivax;    pvmsp-1;    pvmsp-3α;    pvcsp;    Anhui;    China;   

DOI  :  10.1186/1475-2875-13-13

 received in 2013-07-16, accepted in 2013-12-19,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundAlthough the numbers of malaria cases in China have been declining in recent years, outbreaks of Plasmodium vivax malaria were still being reported in rural areas south of the Yellow River. To better understand the transmission dynamics of P. vivax parasites in China, the extent of genetic diversity of P. vivax populations circulating in Bozhou of Anhui province of China were investigated using three polymorphic genetic markers: merozoite surface proteins 1 and 3α (pvmsp-1 and pvmsp-3α) and circumsporozoite protein (pvcsp).MethodsForty-five P. vivax clinical isolates from Bouzhou of Anhui province were collected from 2009 to 2010 and were analysed using PCR/RFLP or DNA sequencing.ResultsSeven and six distinct allelic variants were identified using PCR/RFLP analysis of pvmsp-3α with Hha I and Alu I, respectively. DNA sequence analysis of pvmsp-1 (variable block 5) revealed that there were Sal-I and recombinant types but not Belem type, and seven distinct allelic variants in pvmsp-1 were detected, with recombinant subtype 2 (R2) being predominant (66.7%). All the isolates carried pvcsp with VK210 type but not VK247 or P. vivax-like types in the samples. Sequence analysis of pvcsp gene revealed 12 distinct allelic variants, with VK210-1 being predominant (41.5%).ConclusionsThe present data indicate that there is some degree of genetic diversity among P. vivax populations in Anhui province of China. The genetic data obtained may assist in the surveillance of P. vivax infection in endemic areas or in tracking potential future disease outbreak.

【 授权许可】

Unknown   
© Huang et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.