Genomic variation in two gametocyte non-producing Plasmodium falciparum clonal lines

Malaria Journal

Research

Al C. Ivens¹  Pietro Alano²  Christian Flueck³  Eloise Thompson³  David A. Baker³  Ernest Diez Benavente³  Zaria Gorvett³  Catherine J. Taylor³  Laura G. Drought³  Susana Campino³  Samuel Assefa³  Taane G. Clark⁴  Celine K. Carret⁵  Dominic P. Kwiatkowski⁶ 

[1] Centre for Immunity, Infection and Evolution, University of Edinburgh, Edinburgh, UK;Dipartimento di Malattie Infettive, Parassitarie ed Immunomediate, Istituto Superiore di Sanità, Rome, Italy;Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK;Faculty of Infectious and Tropical Diseases, London School of Hygiene & Tropical Medicine, London, UK;Faculty of Epidemiology and Population Health, London School of Hygiene & Tropical Medicine, London, UK;The European Molecular Biology Organization, Heidelberg, Germany;Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK;Wellcome Trust Sanger Institute, Hinxton, Cambridgeshire, UK;

关键词: Gametocytes;    Plasmodium falciparum;    A4;    F12;    ApiAP2;    Whole genome sequencing;   

DOI  :  10.1186/s12936-016-1254-1

 received in 2016-01-02, accepted in 2016-03-30,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundTransmission of the malaria parasite Plasmodium falciparum from humans to the mosquito vector requires differentiation of a sub-population of asexual forms replicating within red blood cells into non-dividing male and female gametocytes. The nature of the molecular mechanism underlying this key differentiation event required for malaria transmission is not fully understood.MethodsWhole genome sequencing was used to examine the genomic diversity of the gametocyte non-producing 3D7-derived lines F12 and A4. These lines were used in the recent detection of the PF3D7_1222600 locus (encoding PfAP2-G), which acts as a genetic master switch that triggers gametocyte development.ResultsThe evolutionary changes from the 3D7 parental strain through its derivatives F12 (culture-passage derived cloned line) and A4 (transgenic cloned line) were identified. The genetic differences including the formation of chimeric var genes are presented.ConclusionA genomics resource is provided for the further study of gametocytogenesis or other phenotypes using these parasite lines.

【 授权许可】

CC BY   
© Campino et al. 2016