Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage

Journal of Translational Medicine

Research

Ming-Chung Jiang¹  Chun-Chao Chang²  Cheng-Jeng Tai³  Ko-Hung Shen⁴  Tzu-Cheng Su⁵  Chung-Min Yeh⁵  Shu-Hui Lin⁵  Kun-Tu Yeh⁶  Hung-Chang Chen⁷  Woan-Ruoh Lee⁸  Shing-Chuan Shen⁸  Li-Tzu Li⁹  Ching-Fong Liao⁹  Hsin-Yi Shih⁹  Ying-Chun Chen⁹  Ching-Hsiao Lee^1,10 

[1] Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hospital, No.250, Wu-Hsing St., 11031, Taipei, Taiwan;Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hospital, No.250, Wu-Hsing St., 11031, Taipei, Taiwan;Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, No.250, Wu-Hsing St., 11031, Taipei, Taiwan;Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hospital, No.250, Wu-Hsing St., 11031, Taipei, Taiwan;Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, No.250, Wu-Hsing St., 11031, Taipei, Taiwan;Department of Pathology, Changhua Christian Hospital, 135 Nan-Hsiao St., 50006, Changhua, Taiwan;Department of Pathology, Changhua Christian Hospital, 135 Nan-Hsiao St., 50006, Changhua, Taiwan;Jen-Teh Junior College of Medicine, Nursing and Management, No.79-9, Shalunhu, Houlong Township, 35664, Miaoli, Taiwan;Department of Pathology, Changhua Christian Hospital, 135 Nan-Hsiao St., 50006, Changhua, Taiwan;School of Medicine, Chung Shan Medical University, No.110, Sec.1, Jianguo N.Rd., 40201, Taichung, Taiwan;Institute of Clinical Medicine, Kaohsiung Medical University, No.100, Shih-Chuan 1st Road, 80708, Kaohsiung, Taiwan;Division of Colorectal Surgery, Changhua Christian Hospital, No. 135 Nan-Hsiao St., 50006, Changhua, Taiwan;Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, No.252, Wu-Hsing St., 11031, Taipei, Taiwan;Institute of Cellular and Organismic Biology, Academia Sinica, No. 128 Academia Road, Section 2, 11529, Nankang, Taipei, Taiwan;Jen-Teh Junior College of Medicine, Nursing and Management, No.79-9, Shalunhu, Houlong Township, 35664, Miaoli, Taiwan;

关键词: Colorectal cancer;    CSE1L;    Cytoplasm;    Invasion;    Metastasis;    Nucleus;   

DOI  :  10.1186/1479-5876-11-29

 received in 2012-05-10, accepted in 2013-01-16,  发布年份 2013

来源: Springer

【 摘 要 】

BackgroundColorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.MethodsThe invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.ResultsCSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysisConclusionsCSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.

【 授权许可】

Unknown   
© Tai et al.; licensee BioMed Central Ltd. 2013. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.