Correlations between cytoplasmic CSE1L in neoplastic colorectal glands and depth of tumor penetration and cancer stage

Journal of Translational Medicine

Chun-Chao Chang⁷  Hsin-Yi Shih⁵  Ching-Hsiao Lee⁹  Kun-Tu Yeh¹  Chung-Min Yeh⁹  Ko-Hung Shen⁶  Li-Tzu Li⁵  Shu-Hui Lin⁹  Ying-Chun Chen⁵  Ching-Fong Liao⁵  Woan-Ruoh Lee²  Shing-Chuan Shen²  Hung-Chang Chen⁴  Ming-Chung Jiang⁸  Tzu-Cheng Su⁹  Cheng-Jeng Tai³ 

[1] Institute of Clinical Medicine, Kaohsiung Medical University, No.100, Shih-Chuan 1st Road, Kaohsiung 80708, Taiwan;Graduate Institute of Medical Sciences, College of Medicine, Taipei Medical University, No.252, Wu-Hsing St., Taipei 11031, Taiwan;Division of Hematology and Oncology, Department of Internal Medicine, Taipei Medical University Hospital, No.250, Wu-Hsing St., Taipei 11031, Taiwan;Division of Colorectal Surgery, Changhua Christian Hospital, No. 135 Nan-Hsiao St., Changhua 50006, Taiwan;Institute of Cellular and Organismic Biology, Academia Sinica, No. 128 Academia Road, Section 2, Nankang, Taipei 11529, Taiwan;Department of Pathology, Changhua Christian Hospital, 135 Nan-Hsiao St., Changhua, 50006 Taiwan;Division of Gastroenterology and Hepatology, Department of Internal Medicine, Taipei Medical University Hospital, No.250, Wu-Hsing St., Taipei 11031, Taiwan;Department of Internal Medicine, School of Medicine, College of Medicine, Taipei Medical University, Hospital, No.250, Wu-Hsing St., Taipei 11031, Taiwan;Jen-Teh Junior College of Medicine, Nursing and Management, No.79-9, Shalunhu, Houlong Township, Miaoli 35664, Taiwan

关键词: Nucleus;    Metastasis;    Invasion;    Cytoplasm;    CSE1L;    Colorectal cancer;   

Others  :  1205883
DOI  :  10.1186/1479-5876-11-29

 received in 2012-05-10, accepted in 2013-01-16,  发布年份 2013

【 摘 要 】

Background

Colorectal carcinomas spread easily to nearby tissues around the colon or rectum, and display strong potential for invasion and metastasis. CSE1L, the chromosome segregation 1-like protein, is implicated in cancer progression and is located in both the cytoplasm and nuclei of tumor cells. We investigated the prognostic significance of cytoplasmic vs. nuclear CSE1L expression in colorectal cancer.

Methods

The invasion- and metastasis-stimulating activities of CSE1L were studied by in vitro invasion and animal experiments. CSE1L expression in colorectal cancer was assayed by immunohistochemistry, with tissue microarray consisting of 128 surgically resected specimens; and scored using a semiquantitative method. The correlations between CSE1L expression and clinicopathological parameters were analyzed.

Results

CSE1L overexpression was associated with increased invasiveness and metastasis of cancer cells. Non-neoplastic colorectal glands showed minimal CSE1L staining, whereas most colorectal carcinomas (99.2%, 127/128) were significantly positive for CSE1L staining. Cytoplasmic CSE1L was associated with cancer stage (P=0.003) and depth of tumor penetration (P=0.007). Cytoplasmic CSE1L expression also correlated with lymph node metastasis of the disease in Cox regression analysis

Conclusions

CSE1L regulates the invasiveness and metastasis of cancer cells, and immunohistochemical analysis of cytoplasmic CSE1L in colorectal tumors may provide a useful aid to prognosis.

【 授权许可】

   
2013 Tai et al.; licensee BioMed Central Ltd.

【 图 表 】

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