Neuroprotective effect of Demethoxycurcumin, a natural derivative of Curcumin on rotenone induced neurotoxicity in SH-SY 5Y Neuroblastoma cells

BMC Complementary and Alternative Medicine

Research Article

Srinivasagam Rajasankar¹  Arokiasamy Justin Thenmozhi²  Chinnasamy Dhanalakshmi²  Thamilarasan Manivasagam²  Musthafa Mohamed Essa³  Ranganathan Chidambaram⁴  Muthu Ramkumar⁵  Veerappan Venkatesh Gobi⁵  Ameer Kalandar⁶ 

[1] Department of Anatomy, Velammal Medical College and Hospital, 625009, Madurai, Tamil Nadu, India;Department of Biochemistry and Biotechnology, Annamalai University, 608002, Annamalainagar, Tamil Nadu, India;Department of Food Science and Nutrition, CAMS, Sultan Qaboos University, Muscat, Oman;Ageing and Dementia Research Group, Sultan Qaboos University, Muscat, Oman;Food and Brain Research Foundation, 600094, Chennai, Tamil Nadu, India;Department of Radiology, Sri Lakshminarayana Institute of Medical Sciences, Puducherry, India;Research Scholar, Bharath University, Selaiyur, 600073, Chennai, Tamil Nadu, India;School of Medicine, College of Health and Medical Sciences, Haramaya University, Dire Dawa, Ethiopia;

关键词: Demethoxycurcumin;    Rotenone;    Oxidative stress;    Apoptosis;    Neurodegenerative diseases;   

DOI  :  10.1186/s12906-017-1720-5

 received in 2016-10-25, accepted in 2017-04-04,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundMitochondrial dysfunction and oxidative stress are the main toxic events leading to dopaminergic neuronal death in Parkinson’s disease (PD) and identified as vital objective for therapeutic intercession. This study investigated the neuro-protective effects of the demethoxycurcumin (DMC), a derivative of curcumin against rotenone induced neurotoxicity.MethodsSH-SY5Y neuroblastoma cells are divided into four experimental groups: untreated cells, cells incubated with rotenone (100 nM), cells treated with DMC (50 nM) + rotenone (100 nM) and DMC alone treated. 24 h after treatment with rotenone and 28 h after treatment with DMC, cell viability was assessed using the MTT assay, and levels of ROS and MMP, plus expression of apoptotic protein were analysed.ResultsRotenone induced cell death in SH-SY5Y cells was significantly reduced by DMC pretreatment in a dose-dependent manner, indicating the potent neuroprotective effects of DMC. Rotenone treatment significantly increases the levels of ROS, loss of MMP, release of Cyt-c and expression of pro-apoptotic markers and decreases the expression of anti-apoptotic markers.ConclusionsEven though the results of the present study indicated that the DMC may serve as a potent therapeutic agent particularly for the treatment of neurodegenerative diseases like PD, further pre-clinical and clinical studies are required.

【 授权许可】

CC BY   
© The Author(s). 2017