Reclassification of the taxonomic status of SEMIA3007 isolated in Mexico B-11A Mex as Rhizobium leguminosarum bv. viceae by bioinformatic tools

BMC Microbiology

Research Article

Camila Cesário Fernandes¹  Wellington Pine Omori¹  João Carlos Campanharo¹  Luciano Takeshi Kishi¹  Eliana Gertrudes de Macedo Lemos¹ 

[1]Departamento de Tecnologia, Laboratório de Bioquímica de Microrganismos e Planta – LBMP, UNESP - Universidade Estadual Paulista, Faculdade de Ciências Agrárias e Veterinárias, Via de Acesso Prof. Paulo Donato Castellane s/n, 14884-900, Jaboticabal, SP, Brazil

关键词: Genome sequencing;    Core genome;    Comparative analysis;    Ortholog genes;    Phylogenetic analysis;   

DOI  :  10.1186/s12866-016-0882-5

 received in 2016-07-29, accepted in 2016-10-28,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundEvidence based on genomic sequences is extremely important to confirm the phylogenetic relationships within the Rhizobium group. SEMIA3007 was analyzed within the Mesorhizobium groups to define the underlying causes of taxonomic identification. We previously used biochemical tests and phenotypic taxonomic methods to identify bacteria, which can lead to erroneous classification. An improved understanding of bacterial strains such as the Mesorhizobium genus would increase our knowledge of classification and evolution of these species.ResultsIn this study, we sequenced the complete genome of SEMIA3007 and compared it with five other Mesorhizobium and two Rhizobium genomes. The genomes of isolated SEMIA3007 showed several orthologs with M. huakuii, M. erdmanii and M. loti. We identified SEMIA3007 as a Mesorhizobium by comparing the 16S rRNA gene and the complete genome.ConclusionOur ortholog, 16S rRNA gene and average nucleotide identity values (ANI) analysis all demonstrate SEMIA3007 is not Rhizobium leguminosarum bv. viceae. The results of the phylogenetic analysis clearly show SEMIA3007 is part of the Mesorhizobium group and suggest a reclassification is warranted.

【 授权许可】

CC BY   
© The Author(s). 2016