Whole-genome sequencing of Mesorhizobium huakuii 7653R provides molecular insights into host specificity and symbiosis island dynamics

BMC Genomics

Youguo Li³  Binguang Ma¹  Nansheng Chen²  Christian Frech²  Xinyin Zhao²  Fuli Xie³  Jieli Peng³  Huilin Gu³  Jiarui Li²  Baohai Hao¹  Shanming Wang³ 

[1]Center for Bioinformatics, School of Life Science and Technology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China

[2]Department of Molecular Biology and Biochemistry, Simon Fraser University, Burnaby, British Columbia, Canada

[3]State Key Laboratory of Agricultural Microbiology, Huazhong Agricultural University, Wuhan, Hubei, P. R. China

关键词: Symbiosis island;    Host specificity;    Comparative analysis;    Genome sequencing;    Mesorhizobium huakuii 7653R;   

Others  :  1216661
DOI  :  10.1186/1471-2164-15-440

 received in 2013-11-15, accepted in 2014-05-20,  发布年份 2014

【 摘 要 】

Background

Evidence based on genomic sequences is urgently needed to confirm the phylogenetic relationship between Mesorhizobium strain MAFF303099 and M. huakuii. To define underlying causes for the rather striking difference in host specificity between M. huakuii strain 7653R and MAFF303099, several probable determinants also require comparison at the genomic level. An improved understanding of mobile genetic elements that can be integrated into the main chromosomes of Mesorhizobium to form genomic islands would enrich our knowledge of how genome dynamics may contribute to Mesorhizobium evolution in general.

Results

In this study, we sequenced the complete genome of 7653R and compared it with five other Mesorhizobium genomes. Genomes of 7653R and MAFF303099 were found to share a large set of orthologs and, most importantly, a conserved chromosomal backbone and even larger perfectly conserved synteny blocks. We also identified candidate molecular differences responsible for the different host specificities of these two strains. Finally, we reconstructed an ancestral Mesorhizobium genomic island that has evolved into diverse forms in different Mesorhizobium species.

Conclusions

Our ortholog and synteny analyses firmly establish MAFF303099 as a strain of M. huakuii. Differences in nodulation factors and secretion systems T3SS, T4SS, and T6SS may be responsible for the unique host specificities of 7653R and MAFF303099 strains. The plasmids of 7653R may have arisen by excision of the original genomic island from the 7653R chromosome.

【 授权许可】

   
2014 Wang et al.; licensee BioMed Central Ltd.

【 图 表 】

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