In vitro Anti-Toxoplasma gondii Activity of Root Extract/Fractions of Eurycoma longifolia Jack

BMC Complementary and Alternative Medicine

Research Article

Nowroji Kavitha¹  Sreenivasan Sasidharan¹  Rahmah Noordin¹  Kit-Lam Chan² 

[1] Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia, 11800 USM, Pulau Pinang, Malaysia;School of Pharmaceutical Sciences, Universiti Sains Malaysia, USM 11800, Pulau Pinang, Malaysia;

关键词: Toxoplasma gondii;    Eurycoma longifolia;    Antiparasite;    Toxoplasmosis;    Toxoplamacidal activity;   

DOI  :  10.1186/1472-6882-12-91

 received in 2012-02-10, accepted in 2012-06-28,  发布年份 2012

来源: Springer

【 摘 要 】

BackgroundToxoplasma gondii infection causes toxoplasmosis, an infectious disease with worldwide prevalence. The limited efficiency of drugs against this infection, their side effects and the potential appearance of resistant strains make the search of novel drugs an essential need. We examined Eurycoma longifolia root extract and fractions as potential sources of new compounds with high activity and low toxicity. The main goal of this study was to investigate the anti-T. gondii activity of crude extract (TACME) and four fractions (TAF 273, TAF 355, TAF 191 and TAF 401) from E. longifolia, with clindamycin as the positive control.MethodsIn vitro toxoplasmacidal evaluation was performed using Vero cells as host for T. gondii. Light microscopy technique was used to study in situ antiparasitic activity.ResultsSignificant anti-T. gondii activity was observed with clindamycin (EC50 = 0.016 μg/ml), follow by TAF 355 (EC50 = 0.369 μg/ml) and TAF 401 (EC50 = 0.882 μg/ml). Light microscopy revealed that most Vero cells were infected after 3 h of exposure to T. gondii. After 36 h of exposure to the E. longifolia fraction, the host Vero cells showed no visible intracellular parasite and no remarkable morphological changes.ConclusionsOur study demonstrated that TAF 355 and TAF401 fractions may be the sources of new anti-T. gondii compounds.

【 授权许可】

Unknown   
© Kavitha et al.; licensee BioMed Central Ltd. 2012. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.