Effect of methanol extract of Dicranopteris linearis against carbon tetrachloride- induced acute liver injury in rats

BMC Complementary and Alternative Medicine

Research Article

Mohd Khairi Hussain¹  Farhana Yahya¹  Mohamad Fauzi Fahmi Kamarolzaman¹  Siti Syariah Mamat¹  Farah Hidayah Kamisan¹  Zainul Amiruddin Zakaria²  Norhafizah Mohtarrudin³  Mohd Zaki Salleh⁴  Teh Lay Kek⁴ 

[1] Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia;Department of Biomedical Science, Faculty of Medicine and Health Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia;Integrative Pharmacogenomics Institute (iPROMISE), Level 7, FF3, Universiti Teknologi MARA, Puncak Alam Campus, 42300, Puncak Alam, Selangor, Malaysia;Department of Pathology, Faculty of Medicine & Health Sciences, Universiti Putra Malaysia, 43400, UPM Serdang, Selangor, Malaysia;Integrative Pharmacogenomics Institute (iPROMISE), Level 7, FF3, Universiti Teknologi MARA, Puncak Alam Campus, 42300, Puncak Alam, Selangor, Malaysia;

关键词: Dicranopteris linearis;    Methanol extract;    Carbon tetrachloride;    Antioxidant;    Hepatoprotective effect;   

DOI  :  10.1186/1472-6882-14-123

 received in 2013-10-10, accepted in 2014-03-25,  发布年份 2014

来源: Springer

【 摘 要 】

BackgroundDicranopteris linearis (family Gleicheniaceae) has been reported to possess anti-inflammatory and antioxidant activities but no attempt has been made to study its hepatoprotective potential. The aim of the present study was to determine the hepatoprotective effect of methanol extracts of D. linearis (MEDL) against carbon tetrachloride (CCl4)-induced acute liver injury in rats.Methods6 groups (n = 6) of rats received oral test solutions: 10% dimethyl sulfoxide (DMSO), 200 mg/kg silymarin, or MEDL (50, 250, and 500 mg/kg), once daily for 7 consecutive days, followed by hepatotoxicity induction with CCl4. Blood and liver were collected for biochemical and microscopic analysis. The extract was also subjected to antioxidant studies (e.g. 2, 2-diphenyl-1-picrylhydrazyl (DPPH)- and superoxide anion-radical scavenging assays, oxygen radical absorbance capacity (ORAC) test and total phenolic content (TPC) determination), phytochemical screening and HPLC analysis.ResultsPretreatment with MEDL and silymarin significantly (P < 0.05) reduced the serum levels of AST, ALT and ALP, which were increased significantly (P < 0.05) in DMSO-pretreated group following treatment with CCl4. Histological analysis of liver tissues in groups pretreated with MEDL and silymarin showed mild necrosis and inflammation of the hepatocytes compared to the DMSO-pretreated group (negative control group). The MEDL showed higher DPPH- and superoxide anion-radical scavenging activity as well as high TPC and ORAC values indicating high antioxidant activity.ConclusionsMEDL exerts hepatoprotective activity that could be partly contributed by its antioxidant activity and high phenolic content, and hence demands further investigation.

【 授权许可】

Unknown   
© Kamisan et al.; licensee BioMed Central Ltd. 2014. This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.