Sorokiniol: a new enzymes inhibitory metabolite from fungal endophyte Bipolaris sorokiniana LK12

BMC Microbiology

Research Article

Javid Hussain¹  Sang-Mo Kang²  In-Jung Lee²  Muhammad Waqas³  Ahmed Al-Rawahi⁴  Liaqat Ali⁴  Ahmed Al-Harrasi⁴  Abdul Latif Khan⁴ 

[1] Department of Biological Sciences and Chemistry, College of Arts and Sciences, University of Nizwa, Birkat Al-Mouz, 616, Nizwa, Sultanate of Oman;School of Applied Biosciences, Kyungpook National University, 41566, Daegu, Republic of Korea;School of Applied Biosciences, Kyungpook National University, 41566, Daegu, Republic of Korea;Department of Agriculture, Abdul Wali Khan University, Mardan, Pakistan;UoN Chair of Oman’s Medicinal Plants and Marine Natural Products, University of Nizwa, 616, Nizwa, Sultanate of Oman;

关键词: Endophytic fungi;    Secondary metabolites;    Bipolaris sorokiniana;    Cyclic peptides;    Structure elucidation;    Enzyme inhibition and antioxidant;   

DOI  :  10.1186/s12866-016-0722-7

 received in 2015-03-22, accepted in 2016-06-01,  发布年份 2016

来源: Springer

【 摘 要 】

BackgroundMedicinal plants harboring endophytic fungi could carry significant potential for producing bioactive secondary metabolites. Endophytic fungi serve as alternate source of interesting compounds in their natural and modified synthetic forms to treat different diseases. In this regard, endophytic microflora associated with alkaloid-rich medicinal plants Rhazya stricta is least known.ResultsWe isolated one new bioactive compound sorokiniol (1) along with two known cyclic peptides BZR-cotoxin I (2) and BZR-cotoxin IV (3) from fungal endophyte Bipolaris sorokiniana LK12. The structures of the isolated new and known compounds were elucidated through spectroscopic data, including 1D and 2D NMR (1H, 13C, HSQC, HMBC, and NOESY), mass, and UV. The known peptides (2–3) were characterized by ESI-MS, MS/MS, and by comparing the NMR data with the literature. The isolated metabolites were assayed for their role against enzyme inhibition. Compound 1 was significantly inhibitory towards acetyl cholinestrase while the other compounds (2–3) had moderate anti-lipid peroxidation and urease activities.ConclusionThe present results suggest that the endophytic microorganism associated with indigenously important medicinal plants can offer a rich source of biologically active chemical constituents which could help in discovering enzyme inhibitory lead drugs.

【 授权许可】

CC BY   
© The Author(s). 2016