Glucanase and Chitinase from Some Isolates of Endophytic Fungus Trichoderma spp.

International Conference on Chemistry and Material Science 2017

化学;材料科学

Prasetyawan, Sasangka^1 ; Sulistyowati, Lilik^2 ; Aulanni'Am^1

Faculty of Mathematics and Natural Sciences, Brawijaya University, Indonesia^1

Faculty of Agriculture, Brawijaya University, Indonesia^2

关键词: Chitinase activities;    Citrus plant;    Endophytic fungi;    Host plants;    Pathogenic fungi;    Plant tissues;    Stem tissues;    Trichoderma spp;   

Others  :  https://iopscience.iop.org/article/10.1088/1757-899X/299/1/012026/pdf
DOI  :  10.1088/1757-899X/299/1/012026

学科分类：材料科学（综合）

来源: IOP

【 摘 要 】

Endophytic fungi are those fungi that are able to grow in plant tissue without causing symptoms of disease. It is thought that these fungi may confer on the host plants degree of resistance to parasitic invasion. Endophytic fungi have been isolated from stem tissue and these fungi are known to be antagonistic to pathogenic fungi. These endophytes produce chitinase and β-1,3-glucanase enzymes. Based on the fact that chitin and β-1,3-glucan are the main skeletal polysaccharides of the cell walls of fungal patogen. The aim of this research is to do potential test on some of isolates of Trichoderma's endophytic (L-1, L-2, Is-1, Is-2 and Is-7) in the chitinase and β-1,3-glucanase activity in effort to determine endophytic which be chossen to be gene resource for the next research. The gene will be transformed to citrus plant japanese citroen in effort to make citrus plant transgenic resistance to phytopatogenic invasion. The result of this research is endofit namely L-1 is the most potential endophytic fungi with chitinase activities is 4,8 10-2Unit and glucanase 24,2. 1012Unit. The addition of chitin and cell wall of phytophtora causes chitinase activity significantly increase, and also addition of laminarin and cell wall of phytophtora makes glucanase activity increase.