Proposed phase 2/ step 2 in-vitro test on basis of EN 14561 for standardised testing of the wound antiseptics PVP-iodine, chlorhexidine digluconate, polihexanide and octenidine dihydrochloride

BMC Infectious Diseases

Technical Advance

Simon Classen¹  Torsten Koburger²  Ojan Assadian³  Gerald Müller⁴  Kathrin Schedler⁴  Uta Brautferger⁴  Axel Kramer⁴ 

[1] Department for Vascular Surgery, Kerckhoff-Klinik GmbH, Bad Nauheim, Germany;Hygiene Nord GmbH, Greifswald, Germany;Institute for Sin Integrity and Infection Prevention, School of Human and Health Sciences, University of Huddersfield, R1/29 Ramsden Building, Queensgate, HD1 3DH, Huddersfield, UK;Institute of Hygiene and Environmental Medicine, University Medicine, Greifswald, Germany;

关键词: PVP-iodine;    Chlorhexidine digluconate;    Polyhexanide;    Octenidine dihydrochloride;    EN 14561;    Bactericidal efficacy;    Yeasticidal efficacy;    Wound antisepsis;   

DOI  :  10.1186/s12879-017-2220-4

 received in 2016-03-15, accepted in 2017-01-24,  发布年份 2017

来源: Springer

【 摘 要 】

BackgroundCurrently, there is no agreed standard for exploring the antimicrobial activity of wound antiseptics in a phase 2/ step 2 test protocol. In the present study, a standardised in-vitro test is proposed, which allows to test potential antiseptics in a more realistically simulation of conditions found in wounds as in a suspension test. Furthermore, factors potentially influencing test results such as type of materials used as test carrier or various compositions of organic soil challenge were investigated in detail.MethodsThis proposed phase 2/ step 2 test method was modified on basis of the EN 14561 by drying the microbial test suspension on a metal carrier for 1 h, overlaying the test wound antiseptic, washing-off, neutralization, and dispersion at serial dilutions at the end of the required exposure time yielded reproducible, consistent test results.ResultsThe difference between the rapid onset of the antiseptic effect of PVP-I and the delayed onset especially of polihexanide was apparent. Among surface-active antimicrobial compounds, octenidine was more effective than chlorhexidine digluconate and polihexanide, with some differences depending on the test organisms. However, octenidine and PVP-I were approximately equivalent in efficiency and microbial spectrum, while polihexanide required longer exposure times or higher concentrations for a comparable antimicrobial efficacy.ConclusionOverall, this method allowed testing and comparing differ liquid and gel based antimicrobial compounds in a standardised setting.

【 授权许可】

CC BY   
© The Author(s). 2017